PDF(Pubmed)
Abstract：
In understanding stress response mechanisms in fungi, cold stress has received less attention than heat stress. However, cold stress has shown its importance in various research fields. The following study examined the cold stress response of six Pseudogymnoascus spp. isolated from various biogeographical regions through a proteomic approach. In total, 2541 proteins were identified with high confidence. Gene Ontology enrichment analysis showed diversity in the cold stress response pathways for all six Pseudogymnoascus spp. isolates, with metabolic and translation-related processes being prominent in most isolates. 25.6% of the proteins with an increase in relative abundance were increased by more than 3.0-fold. There was no link between the geographical origin of the isolates and the cold stress response of Pseudogymnoascus spp. However, one Antarctic isolate, sp3, showed a distinctive cold stress response profile involving increased flavin/riboflavin biosynthesis and methane metabolism. This Antarctic isolate (sp3) was also the only one that showed decreased phospholipid metabolism in cold stress conditions. This work will improve our understanding of the mechanisms of cold stress response and adaptation in psychrotolerant soil microfungi, with specific attention to the fungal genus Pseudogymnoascus.
摘要：
在了解真菌的应激反应机制时，冷应激比热应激受到的关注要少。然而,冷胁迫在各个研究领域都显示了其重要性。以下研究检查了六个假木曲菌的冷应激反应。通过蛋白质组学方法从各种生物地理区域分离。总的来说,以高置信度鉴定了2541种蛋白质。基因本体论富集分析显示，所有六个假木曲菌种的冷应激反应途径具有多样性。隔离物,代谢和翻译相关的过程在大多数分离物中是突出的。25.6％的相对丰度增加的蛋白质增加了3.0倍以上。分离株的地理起源与假赤霉属的冷应激反应之间没有联系。然而,一个南极隔离，sp3显示出独特的冷应激反应特征，涉及增加的黄素/核黄素生物合成和甲烷代谢。这种南极分离物（sp3）也是唯一在冷胁迫条件下显示磷脂代谢降低的分离物。这项工作将提高我们对耐寒土壤微真菌冷应激反应和适应机制的理解，特别注意真菌属假木曲。
