蛋白质组学在真菌学领域得到了广泛的应用,主要是试图了解与真菌分子功能有关的蛋白质-蛋白质相互作用的复杂网络。它也是比较属内代谢差异的有用工具。假木曲的种类,子囊门下的一个属,已被证明在土壤环境中起着重要作用。它们已在极地和温带地区发现,并且是许多导致土壤分解的细胞外水解酶的已知生产者。尽管假木曲属明显的重要性。在土壤生态系统中,对其分子功能的研究仍然非常有限。在本研究中,6种假赤霉属的蛋白质组学特征。与三个生物地理区域(北极,南极,和温带地区)使用串联质谱法进行。在蛋白质组学分析之前,对蛋白质提取进行了优化。三氯乙酸-丙酮-苯酚被发现是用于伪红曲霉属蛋白质组学分析的最佳提取方法。蛋白质组学分析鉴定了成功映射到UniProtKB数据库的2003蛋白质。鉴定的蛋白质根据其生物学过程和分子功能进行聚类。在所有假木曲菌中发现的共享蛋白质。(1201蛋白质)在它们的基本细胞功能中显示出明显的密切关系,尽管形态结构不同。假树曲菌属的分析。蛋白质组还鉴定了每个区域独特的蛋白质。然而,大量这些蛋白质属于具有相似分子功能的蛋白质家族,即,转移酶和水解酶。我们的蛋白质组数据可用作假木曲菌属的参考。跨全球不同地区,为未来土壤生态系统功能研究奠定了基础。
Proteomics has been used extensively in the field of mycology, mainly in trying to understand the complex network of protein-protein interactions that has been implicated in the molecular functions of fungi. It is also a useful tool to compare metabolic differences within a genus. Species of Pseudogymnoascus, a genus under the phyla Ascomycota, have been shown to play an important role in the soil environment. They have been found in both polar and temperate regions and are a known producer of many extracellular hydrolases that contribute to soil decomposition. Despite the apparent importance of Pseudogymnoascus spp. in the soil ecosystem, investigations into their molecular functions are still very limited. In the present study, proteomic characterization of six Pseudogymnoascus spp. isolated from three biogeographic regions (the Arctic, Antarctic, and temperate regions) was carried out using tandem mass spectrometry. Prior to proteomic analysis, the optimization for protein extraction was carried out. Trichloroacetic acid‑acetone‑phenol was found to be the best extraction method to be used for proteomic profiling of Pseudogymnoascus spp. The proteomic analysis identified 2003 proteins that were successfully mapped to the UniProtKB database. The identified proteins were clustered according to their biological processes and molecular functions. The shared proteins found in all Pseudogymnoascus spp. (1201 proteins) showed a significantly close relationship in their basic cellular functions, despite differences in morphological structures. Analysis of Pseudogymnoascus spp. proteome also identified proteins that were unique to each region. However, a high number of these proteins belonged to protein families of similar molecular functions, namely, transferases and hydrolases. Our proteomic data can be used as a reference for Pseudogymnoascus spp. across different global regions and a foundation for future soil ecosystem function research.