Abstract：
OBJECTIVE: The aim of the study was to assess lameness in dogs with advanced osteoarthritis of the hip and knee joints after a single autologous point-of-care transplantation of the Stromal Vascular Fraction (SVF) into the affected joint.
METHODS: During a minilaparotomy, 10 g of falciform fat was removed from each patient for each joint to be treated. A modern and time-saving procedure (ARC TM System, InGeneron GmbH, Houston, USA) was used for the in-house preparation of the SVF, so that the isolated cells could be applied to the respective joint within 2 h after fat removal. In total, five knee joints of five patients and seven hip joints of four patients were treated.
RESULTS: Improvement in lameness according to owner questionnaires was seen in 3 of 5 patients with knee joint arthritis and 2 of 4 patients with hip joint arthritis. Based on gait analysis, only one dog with gonarthrosis and one dog with coxarthrosis showed improvement up to a maximum of 3 months after surgery.
CONCLUSIONS: This is the first case series on the treatment of osteoarthrosis of the knee or hip joint using point-of-care transplantation of the SVF. In individual cases, this method may represent a therapeutic approach for the treatment in dogs with advanced cox- or gonarthrosis, although only a short-term effect can be expected, which calls into question the effort and costs involved.
METHODS: During a minilaparotomy, 10 g of falciform fat was removed from each patient for each joint to be treated. A modern and time-saving procedure (ARC TM System, InGeneron GmbH, Houston, USA) was used for the in-house preparation of the SVF, so that the isolated cells could be applied to the respective joint within 2 h after fat removal. In total, five knee joints of five patients and seven hip joints of four patients were treated.
RESULTS: Improvement in lameness according to owner questionnaires was seen in 3 of 5 patients with knee joint arthritis and 2 of 4 patients with hip joint arthritis. Based on gait analysis, only one dog with gonarthrosis and one dog with coxarthrosis showed improvement up to a maximum of 3 months after surgery.
CONCLUSIONS: This is the first case series on the treatment of osteoarthrosis of the knee or hip joint using point-of-care transplantation of the SVF. In individual cases, this method may represent a therapeutic approach for the treatment in dogs with advanced cox- or gonarthrosis, although only a short-term effect can be expected, which calls into question the effort and costs involved.
摘要：
目的：本研究的目的是评估犬在将基质血管部分（SVF）进行单次自体护理点移植后，髋关节和膝关节晚期骨关节炎的跛行。
方法：在开腹手术中，对于每个待治疗的关节,从每个患者中取出IOg的镰状脂肪。一个现代和节省时间的程序(ARCTM系统，InGeneronGmbH,休斯顿,美国)用于SVF的内部制备，以便分离的细胞可以在脂肪去除后2小时内应用于各自的关节。总的来说,治疗了5例患者的5个膝关节和4例患者的7个髋关节。
结果：根据所有者问卷调查，5例膝关节关节炎患者中有3例，4例髋关节关节炎患者中有2例，跛行得到改善。基于步态分析,只有一只患有膝关节病的狗和一只患有髋关节病的狗在手术后最多3个月表现出改善。
结论：这是第一个使用SVF的定点护理移植治疗膝关节或髋关节骨关节病的病例系列。在个别情况下,这种方法可能是一种治疗晚期髋关节或膝关节病的治疗方法，尽管只能预期短期效果，这让人质疑所涉及的努力和成本。
方法：在开腹手术中，对于每个待治疗的关节,从每个患者中取出IOg的镰状脂肪。一个现代和节省时间的程序(ARCTM系统，InGeneronGmbH,休斯顿,美国)用于SVF的内部制备，以便分离的细胞可以在脂肪去除后2小时内应用于各自的关节。总的来说,治疗了5例患者的5个膝关节和4例患者的7个髋关节。
结果：根据所有者问卷调查，5例膝关节关节炎患者中有3例，4例髋关节关节炎患者中有2例，跛行得到改善。基于步态分析,只有一只患有膝关节病的狗和一只患有髋关节病的狗在手术后最多3个月表现出改善。
结论：这是第一个使用SVF的定点护理移植治疗膝关节或髋关节骨关节病的病例系列。在个别情况下,这种方法可能是一种治疗晚期髋关节或膝关节病的治疗方法，尽管只能预期短期效果，这让人质疑所涉及的努力和成本。
