Abstract：
BACKGROUND: Fagara zanthoxyloides Lam., an African traditional medicinal plant, is used for treatment of malaria and diabetes.
OBJECTIVE: To investigate the antidiabetic property of ethyl acetate fraction of F. zanthoxyloides root-bark (EAFFZRB) on alloxan-induced diabetic rats.
METHODS: Extraction, isolation, preliminary phytochemical analysis, and acute toxicity study of ethanol extract and fractions of F. zanthoxyloides root-bark were achieved using standard methods. Phyto-constituents in EAFFZRB were identified using HPLC technique. Forty-eight male Wistar rats (140-185 g) were randomized into 6 groups (n = 8). Groups 1 and 2 served as normal and negative controls, respectively. Diabetes was induced in test groups (2-6) using 150 mg/kg body weight (b.w) Alloxan monohydrate. Rats in groups 4-6 received of 200, 400 and 600 mg/kg b.w. EAFFZRB orally, respectively, for 21 days. Group 3 rats received 5 mg/kg b.w Glibenclamide. The effect of EAFFZRB on alterations in hematological, biochemical, and histological indices of study rats were assessed.
RESULTS: Extraction of 3500 g ethanol extract yielded 15.71 g EAFFZRB. HPLC fingerprint of EAFFZRB indicated presence of luteolin, rutin, quercetin, apigenin, cinnamic acid and catechin. Diabetes triggered significant (p < 0.05) alterations in b.w., hematological, biochemical and histological indices of test rats relative to normal control. Treatment with EAFFZRB (LD50 = 3807.9 mg/kg b.w.) resulted in remarkable improvements in altered b.w. changes, hematological, biochemical and histological parameters of diabetic rats.
CONCLUSIONS: The study demonstrated the antidiabetic potential of EAFFZRB, providing scientific basis for traditional use of the plant in treatment of diabetes and its complications.
OBJECTIVE: To investigate the antidiabetic property of ethyl acetate fraction of F. zanthoxyloides root-bark (EAFFZRB) on alloxan-induced diabetic rats.
METHODS: Extraction, isolation, preliminary phytochemical analysis, and acute toxicity study of ethanol extract and fractions of F. zanthoxyloides root-bark were achieved using standard methods. Phyto-constituents in EAFFZRB were identified using HPLC technique. Forty-eight male Wistar rats (140-185 g) were randomized into 6 groups (n = 8). Groups 1 and 2 served as normal and negative controls, respectively. Diabetes was induced in test groups (2-6) using 150 mg/kg body weight (b.w) Alloxan monohydrate. Rats in groups 4-6 received of 200, 400 and 600 mg/kg b.w. EAFFZRB orally, respectively, for 21 days. Group 3 rats received 5 mg/kg b.w Glibenclamide. The effect of EAFFZRB on alterations in hematological, biochemical, and histological indices of study rats were assessed.
RESULTS: Extraction of 3500 g ethanol extract yielded 15.71 g EAFFZRB. HPLC fingerprint of EAFFZRB indicated presence of luteolin, rutin, quercetin, apigenin, cinnamic acid and catechin. Diabetes triggered significant (p < 0.05) alterations in b.w., hematological, biochemical and histological indices of test rats relative to normal control. Treatment with EAFFZRB (LD50 = 3807.9 mg/kg b.w.) resulted in remarkable improvements in altered b.w. changes, hematological, biochemical and histological parameters of diabetic rats.
CONCLUSIONS: The study demonstrated the antidiabetic potential of EAFFZRB, providing scientific basis for traditional use of the plant in treatment of diabetes and its complications.
摘要：
背景：FagarazanthoxyloidesLam。,一种非洲传统药用植物，用于治疗疟疾和糖尿病。
目的：研究刺五氧嘧啶根树皮（EAFFZRB）乙酸乙酯部分对四氧嘧啶诱导的糖尿病大鼠的抗糖尿病作用。
方法：提取，隔离,初步植物化学分析，使用标准方法对乙醇提取物和根皮部分进行了急性毒性研究。使用HPLC技术鉴定了EAFFZRB中的植物成分。将48只雄性Wistar大鼠(140-185g)随机分为6组(n=8)。第1组和第2组作为正常和阴性对照，分别。使用150mg/kg体重在测试组（2-6）中诱导糖尿病（b。w)一水合四氧嘧啶。第4-6组大鼠口服200、400和600mg/kgb.w.EAFFZRB，分别,21天第3组大鼠接受5mg/kgb.w格列本脲。EAFFZRB对血液学改变的影响，生物化学，并对研究大鼠的组织学指标进行评价。
结果:3500g乙醇提取物的提取产生15.71gEAFFZRB。EAFFZRB的HPLC指纹图谱显示木犀草素的存在，芦丁,槲皮素,芹菜素,肉桂酸和儿茶素。糖尿病在b.w.中引发了显着（p<0.05）改变，血液学,相对于正常对照，测试大鼠的生化和组织学指标。用EAFFZRB治疗（LD50=3807.9mg/kgb.w.）导致改变的b.w.变化的显着改善，血液学,糖尿病大鼠的生化和组织学参数。
结论：该研究证明了EAFFZRB的抗糖尿病潜力，为传统应用该植物治疗糖尿病及其并发症提供科学依据。
目的：研究刺五氧嘧啶根树皮（EAFFZRB）乙酸乙酯部分对四氧嘧啶诱导的糖尿病大鼠的抗糖尿病作用。
方法：提取，隔离,初步植物化学分析，使用标准方法对乙醇提取物和根皮部分进行了急性毒性研究。使用HPLC技术鉴定了EAFFZRB中的植物成分。将48只雄性Wistar大鼠(140-185g)随机分为6组(n=8)。第1组和第2组作为正常和阴性对照，分别。使用150mg/kg体重在测试组（2-6）中诱导糖尿病（b。w)一水合四氧嘧啶。第4-6组大鼠口服200、400和600mg/kgb.w.EAFFZRB，分别,21天第3组大鼠接受5mg/kgb.w格列本脲。EAFFZRB对血液学改变的影响，生物化学，并对研究大鼠的组织学指标进行评价。
结果:3500g乙醇提取物的提取产生15.71gEAFFZRB。EAFFZRB的HPLC指纹图谱显示木犀草素的存在，芦丁,槲皮素,芹菜素,肉桂酸和儿茶素。糖尿病在b.w.中引发了显着（p<0.05）改变，血液学,相对于正常对照，测试大鼠的生化和组织学指标。用EAFFZRB治疗（LD50=3807.9mg/kgb.w.）导致改变的b.w.变化的显着改善，血液学,糖尿病大鼠的生化和组织学参数。
结论：该研究证明了EAFFZRB的抗糖尿病潜力，为传统应用该植物治疗糖尿病及其并发症提供科学依据。
