进行这项研究以检查抗氧化活性并评估日期种子粉末kentichi对四氧嘧啶引起的肝脏损伤的保护作用,肾,糖尿病大鼠的胰腺。第1组:对照组,没有接受任何治疗,第2组:腹腔注射四氧嘧啶(120mg/kg体重)2天(Diab),第3组:仅通过饮食中添加的日期种子粉末(300g/kg)处理6周(DSPK),组4:用枣子粉(300g/kg)(DSPK+Diab)处理的四氧嘧啶-糖尿病大鼠。确定血液中生化参数的估计值。TBARS,SOD,CAT,并测定GPx活性。通过将两个器官的碎片浸入固定溶液中,然后进行石蜡苏木精-伊红染色,进行了组织病理学研究。此外,通过DPPH自由基清除活性来评价DSPK的抗氧化活性,降低功率,和ABTS自由基清除。结果表明,日期种子显著降低血清葡萄糖水平,胆固醇,甘油三酯,尿素,肌酐,T蛋白,ALP,D-bili和T-bili水平。此外,超氧化物歧化酶(SOD),过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)活性在肝脏中降低,肾,治疗组的胰腺通过DSPK治疗恢复,因此,肝脏中的脂质过氧化水平降低,与对照组相比,肾脏和胰腺组织。此外,用枣子粉处理后,这些器官的组织学结构得以恢复。
The study was conducted to examine the antioxidant activity and evaluate the protective effects of the date seeds powder kentichi against
alloxan-induced damage in the liver, kidney, and pancreas in diabetic\'s rats. Group 1: control group, that did not receive any treatment, Group 2:
alloxan was injected intraperitoneally (120 mg/kg body weight) for two days (Diab), Group 3: treated only by date seeds powder added in the diet (300 g/kg) for 6 weeks (DSPK), Group 4:
alloxan-diabetic rats treated with date seeds powder (300 g/kg) (DSPK + Diab). Estimations of biochemical parameters in blood were determined. TBARS, SOD, CAT, and GPx activities were determined. A histopathological study was done by immersing pieces of both organs in a fixative solution followed by paraffin hematoxylin-eosin staining. In addition, the antioxidant activities of DSPK were evaluated by DPPH radical scavenging activity, reducing power, and ABTS free radical scavenging. The results revealed that date seeds significantly decreased serum levels of glucose, cholesterol, triglycerides, urea, creatinine, T-protein, ALP, D-bili and T-bili levels. In addition, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities that had been reduced in liver, kidney, and pancreas of the treated group were restored by DSPK treatments and, therefore, the lipid peroxidation level was reduced in the liver, kidney and pancreas tissue compared to the control group. Additionally, the histological structure in these organs was restored after treatment with date seeds powder.