PDF(Pubmed)
Abstract：
In this study, we describe an inexpensive and rapid method of using video analysis and identity tracking to measure the effects of tag weight on insect movement. In a laboratory experiment, we assessed the tag weight and associated context-dependent effects on movement, choosing temperature as a factor known to affect insect movement and behavior. We recorded the movements of groups of flightless adult crickets Gryllus locorojo (Orthoptera:Gryllidae) as affected by no tag (control); by light, medium, or heavy tags (198.7, 549.2, and 758.6 mg, respectively); and by low, intermediate, or high temperatures (19.5, 24.0, and 28.3°C, respectively). Each individual in each group was weighed before recording and was recorded for 3 consecutive days. The mean (± SD) tag mass expressed as a percentage of body mass before the first recording was 26.8 ± 3.7% with light tags, 72 ± 11.2% with medium tags, and 101.9 ± 13.5% with heavy tags. We found that the influence of tag weight strongly depended on temperature, and that the negative effects on movement generally increased with tag weight. At the low temperature, nearly all movement properties were negatively influenced. At the intermediate and high temperatures, the light and medium tags did not affect any of the movement properties. The continuous 3-day tag load reduced the average movement speed only for crickets with heavy tags. Based on our results, we recommend that researchers consider or investigate the possible effects of tags before conducting any experiment with tags in order to avoid obtaining biased results.
摘要：
在这项研究中,我们描述了一种廉价且快速的方法，使用视频分析和身份跟踪来测量标签重量对昆虫运动的影响。在实验室实验中,我们评估了标签权重和相关的上下文相关的运动影响，选择温度作为已知影响昆虫运动和行为的因素。我们记录了不受标签（对照）影响的不会飞的成虫Grylluslocorojo（直翅目：Gryllidae）组的运动；通过光，中等,或重标签(198.7、549.2和758.6毫克，分别)；并按低，中间,或高温（19.5、24.0和28.3°C，分别)。每组中的每个个体在记录前称重并连续记录3天。在第一次记录之前，以体重百分比表示的平均（±SD）标签质量为26.8±3.7％，72±11.2%带中等标签,和101.9±13.5％的重标签。我们发现标签重量的影响很大程度上取决于温度，对运动的负面影响通常随着标签重量的增加而增加。在低温下，几乎所有的运动特性都受到了负面影响。在中温和高温下，轻型和中型标签不影响任何运动特性。连续3天的标签负荷仅降低了标签重的the的平均运动速度。根据我们的结果,我们建议研究人员在进行任何标签实验之前考虑或调查标签的可能影响，以避免获得有偏见的结果。
