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Abstract：
Site-specific recombinases (SSRs) are invaluable genome engineering tools that have enormously boosted our understanding of gene functions and cell lineage relationships in developmental biology, stem cell biology, regenerative medicine, and multiple diseases. However, the ever-increasing complexity of biomedical research requires the development of novel site-specific genetic recombination technologies that can manipulate genomic DNA with high efficiency and fine spatiotemporal control. Here, we review the latest innovative strategies of the commonly used Cre-loxP recombination system and its combinatorial strategies with other site-specific recombinase systems. We also highlight recent progress with a focus on the new generation of chemical- and light-inducible genetic systems and discuss the merits and limitations of each new and established system. Finally, we provide the future perspectives of combining various recombination systems or improving well-established site-specific genetic tools to achieve more efficient and precise spatiotemporal genetic manipulation.
摘要：
位点特异性重组酶（SSR）是宝贵的基因组工程工具，极大地促进了我们对发育生物学中基因功能和细胞谱系关系的理解。干细胞生物学，再生医学,和多种疾病。然而,生物医学研究日益复杂,需要开发新的位点特异性基因重组技术,该技术可以高效和精细的时空控制操作基因组DNA.这里,我们回顾了常用Cre-loxP重组系统的最新创新策略及其与其他位点特异性重组酶系统的组合策略。我们还重点介绍了最近的进展，重点是新一代的化学和光诱导遗传系统，并讨论了每个新系统和已建立系统的优点和局限性。最后,我们提供了结合各种重组系统或改进完善的位点特异性遗传工具以实现更有效和精确的时空遗传操作的未来观点。
