背景:组蛋白脱乙酰酶(HDAC)和组蛋白乙酰转移酶(HAT)参与植物的生长和发育以及对环境变化的响应,通过动态调节基因乙酰化水平。尽管已有许多关于HDAC和HAT在草本植物中的鉴定和功能的报道,干旱胁迫下木本植物的相关基因报道较少。
结果:在这项研究中,我们对毛果杨的HDAC和HAT家族进行了全基因组分析,包括系统发育分析,基因结构,保守域,和表达分析。共鉴定出16个PtrHDAC和12个PtrHAT。对PtrHDACs和PtrHATs启动子中的顺式元件的分析表明,这两个基因家族都可以响应多种环境信号,包括荷尔蒙和干旱.此外,实时定量PCR表明PtrHDA906和PtrHAG3对干旱有显著响应。PtrHDA906,PtrHAC1,PtrHAC3,PtrHAG2,PtrHAG6和PtrHAF1对脱落酸的反应一致,干旱条件下的茉莉酸甲酯和水杨酸。
结论:我们的研究表明,PtrHDACs和PtrHATs可能通过激素信号通路对干旱做出反应,这有助于揭示乙酰化修饰在非生物胁迫激素调控中的枢纽。
BACKGROUND: Histone deacetylases (HDACs) and histone acetyltransferases (HATs) are involved in plant growth and development as well as in response to environmental changes, by dynamically regulating gene acetylation levels. Although there have been numerous reports on the identification and function of HDAC and HAT in herbaceous plants, there are fewer report related genes in woody plants under drought stress.
RESULTS: In this study, we performed a genome-wide analysis of the HDAC and HAT families in Populus trichocarpa, including phylogenetic analysis, gene structure, conserved domains, and expression analysis. A total of 16 PtrHDACs and 12 PtrHATs were identified in P. trichocarpa genome. Analysis of cis-elements in the promoters of PtrHDACs and PtrHATs revealed that both gene families could respond to a variety of environmental signals, including hormones and drought. Furthermore, real time quantitative PCR indicated that PtrHDA906 and PtrHAG3 were significantly responsive to drought. PtrHDA906, PtrHAC1, PtrHAC3, PtrHAG2, PtrHAG6 and PtrHAF1 consistently responded to abscisic acid, methyl jasmonate and salicylic acid under drought conditions.
CONCLUSIONS: Our study demonstrates that PtrHDACs and PtrHATs may respond to drought through hormone signaling pathways, which helps to reveal the hub of acetylation modification in hormone regulation of abiotic stress.