关键词： APCs, antigen-presenting cells BMDCs, bone marrow-derived dendritic cells BW, body weight Brassica rapa L. CFSE, carboxyfluorescein diacetate succinimidyl ester CLRs, C-type lectin receptors Con A, concanavalin A Cyto D, cytochalasin D DCs, dendritic cells EDTA, ethylenediamine-N,N,N′,N′,-tetraacetic acid ELISA, enzyme-linked immunosorbent assay ERK, extracellular signal-related kinase FBS, fetal bovine serum FITC, fluorescein isothiocyanate GM-CSF, granulocyte-macrophage colony-stimulating factor IFN, interferon IL, interleukin JNK, jun kinase LPS, lipopolysaccharide MAPK, mitogen-activated protein kinase MHC, major histocompatibility complex NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells NK, natural killer PB, polymyxin B PBS, phosphate-buffered saline PE, phycoerythrin PGN, peptidoglycan PI, propidium iodide PPs, Peyer’s patches RPMI, Roswell Park Memorial Institute SD, standard deviation Syk, spleen tyrosine kinase TCR, T cell receptor TLRs, Toll-like receptors TNF, tumor necrosis factor WT, wild-type dendritic cells i.p., intraperitoneally immunomodulation interferon-γ mAbs, monoclonal antibodies mLNs, mesenteric lymph nodes natural killer cell activity

Mesh ： Adjuvants, Immunologic / administration & dosage pharmacology Administration, Oral Animals Brassica rapa / metabolism Cytokines / metabolism Female Functional Food Interferon-gamma / biosynthesis Interleukin-12 / physiology Killer Cells, Natural / drug effects immunology Lectins, C-Type / metabolism Male Mice, Inbred C57BL Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism Plant Extracts / administration & dosage pharmacology Signal Transduction Spleen / drug effects metabolism Syk Kinase / metabolism Toll-Like Receptor 2 / metabolism Toll-Like Receptor 4 / metabolism

来  源：   DOI：10.1080/09168451.2017.1408396   PDF(Sci-hub)

Abstract：
Certain food components possess immunomodulatory effects. The aim of this study was to elucidate the mechanism of the immunostimulatory activity of Brassica rapa L. We demonstrated an enhancement of natural killer (NK) activity and interferon (IFN)-γ production in mice that were orally administered an insoluble fraction of B. rapa L. The insoluble fraction of B. rapa L. significantly induced IFN-γ production in mouse spleen cells in an interleukin (IL)-12-dependent manner, and NK1.1+ cells were the main cells responsible for producing IFN-γ. Additionally, the results suggested that the active compounds in the insoluble fraction were recognized by Toll-like receptor (TLR) 2, TLR4, and C-type lectin receptors on dendritic cells, and they activated signaling cascades such as MAPK, NF-κB, and Syk. These findings suggest that B. rapa L. is a potentially promising immuno-improving material, and it might be useful for preventing immunological disorders such as infections and cancers by activating innate immunity.
摘要：
某些食物成分具有免疫调节作用。这项研究的目的是阐明甘蓝型油菜的免疫刺激活性的机制。我们证明了口服B.rapaL.的不溶性部分的小鼠的自然杀伤（NK）活性和干扰素（IFN）-γ产生的增强。和NK1.1+细胞是负责产生IFN-γ的主要细胞。此外,结果表明，不溶性部分中的活性化合物被树突状细胞上的Toll样受体（TLR）2，TLR4和C型凝集素受体识别，它们激活了信号级联，如MAPK，NF-κB,还有Syk.这些发现表明，B.rapaL.是一种潜在的有前途的免疫改善材料，它可能有助于通过激活先天免疫来预防免疫疾病，如感染和癌症。
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