UNASSIGNED:人参皂甙是人参的生物活性成分,具有多种功能。在这项研究中,我们研究了通过酶促生物转化从人参粉(GP)产生的人参产品的免疫调节活性。本产品,通用生物化合物K-10mg溶液(GBCK10S),表现出少量人参皂苷的水平增加,包括人参皂苷F1,化合物K,
未经证实:使用小鼠和人自然杀伤(NK)细胞系证实GBCK10S的免疫调节性质。我们通过酶联免疫吸附试验监测参与免疫反应的分子的表达,流式细胞术,NK细胞靶向细胞破坏,定量逆转录实时聚合酶链反应,和蛋白质印迹分析。
UNASSIGNED:口服GBCK10S可显着增加血清免疫球蛋白M水平,并引发脾细胞表达促炎细胞因子,例如白介素6,肿瘤坏死因子-α,和干扰素-γ。口服GBCK10S也激活了小鼠的NK细胞。此外,GBCK10S处理在体外刺激人NK细胞系,从而增加颗粒酶B基因表达并激活STAT5。
UNASSIGNED:GBCK10S可能具有有效的免疫刺激特性,可以激活B细胞介导的免疫反应,Th1型T细胞,NK细胞
UNASSIGNED: Ginsenosides are biologically active components of ginseng and have various functions. In this study, we investigated the immunomodulatory activity of a ginseng product generated from ginseng powder (GP) via enzymatic bioconversion. This product, General Bio compound K-10 mg solution (GBCK10S), exhibited increased levels of minor ginsenosides, including ginsenoside-F1, compound K, and compound Y.
UNASSIGNED: The immunomodulatory properties of GBCK10S were confirmed using mice and a human natural killer (NK) cell line. We monitored the expression of molecules involved in immune responses via enzyme-linked immunosorbent assay, flow cytometry, NK cell-targeted cell destruction, quantitative reverse-transcription real-time polymerase chain reaction, and Western blot analyses.
UNASSIGNED: Oral administration of GBCK10S significantly increased serum immunoglobulin M levels and primed splenocytes to express pro-inflammatory cytokines such as interleukin-6, tumor necrosis factor-α, and interferon-γ. Oral administration of GBCK10S also activated NK cells in mice. Furthermore, GBCK10S treatment stimulated a human NK cell line in vitro, thereby increasing granzyme B gene expression and activating STAT5.
UNASSIGNED: GBCK10S may have potent immunostimulatory properties and can activate immune responses mediated by B cells, Th1-type T cells, and NK cells.