这项研究的目的是评估异齿型软木叶的乙醇提取物的抗氧化特性和相关的药理潜力,传统上用于治疗疼痛,损伤,疟疾热,癫痫和胃酸过多,其次是用于分析由GC-MS鉴定的生物活性化合物的计算方法。在GC-MS分析中,提取物产生了十种化合物,其中4,6-二叔丁基-2-α-甲基苄基苯酚具有最高的量。使用2,2-二苯基-1-吡啶酰肼(DPPH)自由基和过氧化氢清除试验对植物的抗氧化性能进行了体外研究。次生代谢物酚类物质的数量,黄酮类化合物,单宁的含量为142mgGAE/g,534mgQE/g,和110毫克GAE/克,分别。对小鼠进行了急性毒性研究,这表明在4000毫克/千克体重的剂量下没有毒性。对于250和500毫克/千克体重的提取物剂量,乙酸诱导的扭体反应试验对小鼠具有统计学意义(p<0.05)的镇痛作用。口服葡萄糖耐量试验(OGTT)和α-葡萄糖苷酶抑制活性测定用于检查抗高血糖的潜力,其中提取物将血糖水平降低至6.22mmol/l和3.82mmol/l,剂量为250和500毫克/千克体重,分别在OGTT中60分钟,即使在α-葡萄糖苷酶抑制测定中未观察到活性。在抗菌试验中,提取物对大肠杆菌的最低抑菌浓度(MIC),铜绿假单胞菌,金黄色葡萄球菌被确定为8、16和8µg/ml,分别。这项研究表明,在民俗药物中使用A.molissima叶是合理的。
The aim of this study is to assess the antioxidative profile and related pharmacological potentialities of the ethanolic extract of Amischotolype mollissima leaves, traditionally used in treating pain, injury, malarial fever, epilepsy and hyperacidity, followed by a computational approach for the analysis of bioactive compounds identified by GC-MS. In GC-MS analysis, the extract yielded ten compounds, with 4,6-di-t-butyl-2-alpha-methyl benzyl phenol having the highest amount. In vitro investigation of the antioxidative properties of the plant was conducted with 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical and hydrogen peroxide scavenging assays. The amounts of secondary metabolites phenolics, flavonoids, and tannins were measured at 142 mg GAE/g, 534 mg QE/g, and 110 mg GAE/g, respectively. An acute toxicity study was carried out on mice, which revealed no toxicity up to the dosage of 4000 mg/kg bw. For the dosages of extract at 250 and 500 mg/kg bw, the writhing response test induced by acetic acid exhibited a statistically significant (p < 0.05) analgesic effect in mice. The oral glucose tolerance test (OGTT) and alpha-glucosidase enzyme inhibitory activity assay were used to examine the antihyperglycemic potential, in which the extract reduced the blood glucose level to 6.22 mmol/l and 3.82 mmol/l, at dosages of 250 and 500 mg/kg bw, respectively at 60 min in OGTT even though no activity was observed in the α-glucosidase enzyme inhibitory assay. In an antibacterial assay, the extract\'s minimum inhibitory concentration (MIC) against E. coli, P. aeruginosa, and S. aureus was determined to be 8, 16, and 8 µg/ml, respectively. This study shows that the usage of A. mollissima leaves in folklore medication is justified.