UNASSIGNED:免疫系统的功能是保护宿主免受各种传染病的侵害。这里,我们评估了绿咖啡提取物(GCE)的体外免疫调节作用,并进行了双盲,在明显健康的个体中进行随机和安慰剂对照试验。
未经授权:我们确定了炎症和免疫标志物的水平和功能。,磷酸化NF-κBp65ser536,趋化性,吞噬作用,TH1/TH2细胞因子和IgG产生。我们还评估了几种免疫学标记,例如总白细胞计数,区分白细胞计数,NK细胞活性,CD4/CD8比值,血清免疫球蛋白,C反应蛋白(CRP)和促炎细胞因子(IL-6和TNF-α)。
未经证实:GCE显著抑制LPS诱导的NF-κBp65ser536磷酸化,MCP-1诱导趋化性,并显着增强吞噬作用和IgG产生。此外,GCE调节PMA/PHA诱导的TH1/TH2细胞因子产生。临床研究表明,GCE治疗后,NK细胞上CD56和CD16的表达显着增加。GCE在流感疫苗接种前后显着增强了IgA的产生。同样,GCE可显著抑制IL-6、TNF-α和CRP水平。一起,GCE在不同水平上赋予几种有益的免疫调节作用,这归因于宿主中免疫反应的最佳功能。
未经证实:细胞生物学,临床研究,临床试验。
UNASSIGNED: The immune system functions to protect the host from a broad array of infectious diseases. Here, we evaluated the in vitro immunomodulatory effects of green coffee extract (GCE), and conducted a double-blinded, randomized and placebo-controlled trial among apparently healthy individuals.
UNASSIGNED: We determined the levels and functions of inflammatory and immune markers viz., phospho-NF-κB p65 ser536, chemotaxis, phagocytosis, TH1/TH2 cytokines and IgG production. We also evaluated several immunological markers such as total leukocyte counts, differential leukocyte counts, NK cell activity, CD4/CD8 ratio, serum immunoglobulin, C-reactive protein (CRP) and pro-inflammatory cytokines (IL-6 and TNF-α).
UNASSIGNED: GCE significantly inhibited LPS-induced NF-κB p65 ser536 phosphorylation, MCP-1-induced chemotaxis and significantly enhanced phagocytosis and IgG production. In addition, GCE modulated PMA/PHA-induced TH1/TH2 cytokine production. Clinical investigations suggested that the expression of CD56 and CD16 was markedly augmented on NK cells following GCE treatment. GCE significantly enhanced IgA production before and after influenza vaccination. Similarly, IL-6, TNF-α and CRP levels were significantly inhibited by GCE. Together, GCE confers several salubrious immunomodulatory effects at different levels attributing to optimal functioning of immune responses in the host.
UNASSIGNED: Cell biology, Clinical study, Clinical Trial.