未经证实:人骨髓来源的间充质干细胞(hBM-MSCs)是具有自我更新能力和多谱系分化的未分化细胞,对再生医学有益。纳米支架是用于骨修复和再生的新型材料。Nisin是一种益生元,可以增加干细胞的寿命和增殖。本研究试图为Nisin预处理后在聚-L-乳酸(PLLA)支架上骨髓间充质干细胞分化为骨细胞提供适当的策略。
UNASSIGNED:通过测量钙,碱性磷酸酶,和定量测试,如实时PCR,吖啶橙,茜素红,冯·科萨,和其他人。
UNASSIGNED:MTT试验结果表明,1日,MSCs预处理的Nisin益生元的最佳剂量为200IU/mL,3rd,文化的第五天实时PCR数据表明ALP的表达率,骨连蛋白,骨钙蛋白,在Nisin的存在下,我增加了胶原蛋白,而RUNX-2基因表达下降。此外,茜素红和VonKossa测试的结果,以及扫描电子显微镜(SEM),结果表明,用Nisin预处理的样品中的细胞增殖显着增加。
UNASSIGNED:该研究得出结论,在PLLANano支架上用Nisin预处理的样品中,细胞增殖和分化增加。
UNASSIGNED: Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are undifferentiated cells with self-renewing ability and multi-lineage differentiation beneficial for regenerative medicine. Nano scaffolds are novel materials employed in bone repair and regeneration. Nisin is a prebiotic that can increase stem cells\' lifespan and proliferation. This study attempted to provide a proper strategy for bone marrow mesenchymal stem cells differentiation into the Osteocytes on a Poly-L-lactic-acid (PLLA) scaffold after pretreating with Nisin.
UNASSIGNED: MSC osteogenic differentiation was evaluated by measuring Calcium, Alkaline phosphatase, and quantitative tests such as Real-Time PCR, Acridine Orange, Alizarin Red, Von Kossa, and others.
UNASSIGNED: The result of the MTT test showed that the optimal dose of Nisin prebiotic for the MSCs\' preconditioning was 200 IU/mL on the 1st, 3rd, and 5th days of culture. Real-time PCR data indicated that the expression rate of ALP, Osteonectin, Osteocalcin, and Collagen I have increased in the presence of Nisin, while the RUNX-2 gene expression has decreased. Furthermore, the results of Alizarin Red and Von Kossa tests, as well as Scanning electron microscopy (SEM), revealed that the cell proliferation in the preconditioned samples with Nisin increased significantly.
UNASSIGNED: The study concluded that the cell proliferation and differentiation increased in samples pretreated with Nisin on the PLLA Nano scaffolds.