目的:玻璃体液(VH)用于死后生化研究,因为即使在死亡后也能在未污染的状态下得到很好的保护。这项研究的目的是调查有或没有玻璃体切割手术史的人眼中VH中的电解质浓度。
方法:我们分析了钠(Na),钾(K),来自34名患者的34个VH样品的氯化物(Cl)和镁(Mg)浓度。11个样本来自有玻璃体切除术史的眼睛,其余23只眼无玻璃体切除术史。Na的相关性,K,Cl和Mg浓度随患者年龄的变化,第一次和第二次玻璃体切除术之间的间隔,还评估了晶状体状态(白内障手术史).
结果:Na,K,玻璃体切除眼的VH中的Cl和Mg浓度为134.1±7.9mmol/L,3.7±0.2mmol/L,99.7±6.7mmol/L和0.59±0.09mmol/L,分别;均显着低于对照组眼中VH中的相应浓度(降低5.0%,11.0%,11.7%,和22.6%,分别)。Na,K,玻璃体切除眼的VH中的Cl和Mg浓度与患者年龄或第一次和第二次玻璃体切除之间的间隔没有显着相关性。Na、K,有晶状体眼和人工晶状体植入眼之间VH中的Cl和Mg浓度。
结论:随着玻璃体切除术数量的增加,在法医检查中使用受试者的VH时,有必要考虑玻璃体切除术的病史。
OBJECTIVE: Vitreous humor (VH) is used for postmortem biochemical studies because it is well protected in an uncontaminated state even after death. The goal of this research was to investigate electrolyte concentrations in the VH from human eyes with and without a history of vitrectomy surgery.
METHODS: We analyzed the sodium (Na), potassium (K), chloride (Cl) and magnesium (Mg) concentrations from 34 VH samples from 34 patients. Eleven samples were from eyes with a history of vitrectomy, and the remaining 23 eyes had no history of vitrectomy. The correlations of Na, K, Cl and Mg concentrations with patient age, interval between first and second vitrectomy, and lens status (history of cataract surgery) were also evaluated.
RESULTS: The Na, K, Cl and Mg concentrations in VH from vitrectomized eyes were 134.1 ± 7.9 mmol/L, 3.7 ± 0.2 mmol/L, 99.7 ± 6.7 mmol/L and 0.59 ± 0.09 mmol/L, respectively; all were significantly lower than the corresponding concentrations in VH from control eyes (lower by 5.0%, 11.0%, 11.7%, and 22.6%, respectively). Na, K, Cl and Mg concentrations in VH from vitrectomized eyes did not show significant correlations with patient ages or the interval between their first and second vitrectomies. There were no significant differences in Na, K, Cl and Mg concentrations in VH between phakic eyes and intraocular lens-implanted eyes.
CONCLUSIONS: With the increasing number of vitrectomies being performed, it is necessary to consider the history of vitrectomy when using a subject\'s VH in forensic examination.