关键词: Raman spectroscopy bloodstain aging chemometrics menstrual blood time since deposition

Mesh : Humans Spectrum Analysis, Raman / methods Blood Stains Menstruation / blood physiology Female Forensic Medicine / methods Time Factors Adult Forensic Sciences / methods

来  源:   DOI:10.3390/s24113262   PDF(Pubmed)

Abstract:
Forensic chemistry plays a crucial role in aiding law enforcement investigations by applying analytical techniques for the analysis of evidence. While bloodstains are frequently encountered at crime scenes, distinguishing between peripheral and menstrual bloodstains presents a challenge. This is due to their similar appearance post-drying. Raman spectroscopy has emerged as a promising technique capable of discriminating between the two types of bloodstains, offering invaluable probative information. Moreover, estimating the time since deposition (TSD) of bloodstains aids in crime scene reconstruction and prioritizing what evidence to collect. Despite extensive research focusing on TSD estimations, primarily in peripheral bloodstains, a crucial gap exists in determining the TSD of menstrual bloodstains. This study demonstrates how Raman spectroscopy effectively analyzes biological samples like menstrual blood, showing similar aging patterns to those of peripheral blood and provides proof-of-concept models for determining the TSD of menstrual blood. While this work shows promising results for creating a universal model for bloodstain age determination, further testing with more donors needs to be conducted before the implementation of this method into forensic practice.
摘要:
法医化学通过应用分析技术来分析证据,在协助执法调查中起着至关重要的作用。虽然犯罪现场经常遇到血迹,区分外周和经血是一个挑战。这是由于它们在干燥后的相似外观。拉曼光谱已经成为一种有前途的技术,能够区分两种类型的血迹,提供无价的证明性信息。此外,估计血迹沉积(TSD)后的时间有助于犯罪现场重建,并优先考虑收集哪些证据。尽管广泛的研究集中在TSD估计上,主要在外周血迹中,在确定经血的TSD时存在一个关键的差距。这项研究证明了拉曼光谱如何有效地分析月经血等生物样本,显示出与外周血相似的衰老模式,并提供了用于确定月经血TSD的概念验证模型。虽然这项工作在创建血迹年龄确定的通用模型方面显示了有希望的结果,在将该方法应用于法医实践之前,需要对更多的捐助者进行进一步的测试。
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