■头癣(T.capitis),俗称头皮癣,是影响头皮和头发的真菌感染。在病原体中,犬小孢子菌(M.Canis)脱颖而出,经常从猫传播给人类(人畜共患疾病)。在这项研究中,我们调查了番木瓜的功效(C.木瓜),对皮肤癣菌的水果提取物,尤其是M.canis,在体外和体内。此外,我们旨在确定抑制真菌生长的活性化合物,并评估木瓜对人细胞的毒性。
■它分两个部分进行。首先,体外研究包括以甲醇为溶剂制备木瓜果实提取物,植物提取物的化学分析包括气相色谱-质谱(GC-MS)和傅里叶变换红外光谱(FTIR),使用HUH-7细胞进行细胞毒性测定,采用MTT测定(1-(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物),对犬分枝杆菌的抗菌活性进行了评估,包括:抑制区(ZI),最小抑制浓度(MIC),最小杀菌浓度(MFC),使用扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察犬M.canis细胞的改变。第二,在Vivo,包括白化病Wistar雄性大鼠。
■木瓜甲醇提取物的植物化学分析揭示了几个官能团,包括羟基,氨,烷烃,碳酸盐,和酒精。此外,GC-MS分析确定了15种化合物,黄嘌呤和癸酸是主要成分。木瓜果实的甲醇提取物表现出有效的抗真菌活性:ZI=37毫米,MIC=1,000μg/mL,MFC=1900μg/mL,MTT结果表明,在20μg/mL的浓度下,水果提取物的细胞毒性较低,50μg/mL,100μg/mL,150μg/mL,和200μg/mL,IC50显示细胞活力随着提取物浓度的增加而显著降低。值得注意的是,木瓜提取物引起犬分枝杆菌菌丝和孢子形态的明显改变。在动物组织中,在用木瓜提取物治疗的大鼠组中观察到改善。这项研究强调了木瓜果实作为天然抗真菌剂的潜力,保证临床应用的进一步探索。
UNASSIGNED: Tinea capitis (T. capitis), commonly known as scalp ringworm, is a fungal infection affecting the scalp and hair. Among the causative agents, Microsporum canis (M. canis) stands out, often transmitted from cats to humans (zoonotic disease). In this study, we investigated the efficacy of Carica papaya (C. papaya), fruit extract against dermatophytes, particularly M. canis, both in vitro and in vivo. Additionally, we aimed to identify the active compounds responsible for suppressing fungal growth and assess the toxicity of C. papaya on human cells.
UNASSIGNED: It conducted in two parts. First, In Vitro Study include the preparation of C. papaya fruit extract using methanol as the solvent, Phytochemical analysis of the plant extract including Gas chromatography-mass spectrometry (GC-MS) and Fourier-transform infrared spectroscopy (FTIR) was conducted, Cytotoxicity assays were performed using HUH-7 cells, employing the MTT assay (1-(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), Antimicrobial activity against M. canis was evaluated, including: Zone of inhibition (ZI), Minimum inhibitory concentration (MIC), Minimum fungicidal concentration (MFC), M. canis cell alterations were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Second, In Vivo, Albino Wistar male rats were included.
UNASSIGNED: The phytochemical analysis of the methanolic extract from papaya revealed several functional groups, including hydroxyl, ammonia, alkane, carbonate, and alcohol. Additionally, the GC-MS analysis identified 15 compounds, with xanthosine and decanoic acid being the predominant components. The methanolic extract of papaya fruits demonstrated potent antifungal activity: ZI = 37 mm, MIC = 1,000 μg/mL, MFC = 1900 μg/mL, MTT results indicated lower cytotoxicity of the fruit extract at concentrations of 20 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL, and 200 μg/mL, The IC50 revealed a significant decrease in cell viability with increasing extract concentration. Notably, papaya extract induced considerable alterations in the morphology of M. canis hyphae and spores. In animal tissue, improvements were observed among the group of rats which treated with Papaya extract. This study highlights the potential of C. papaya fruits as a natural antifungal agent, warranting further exploration for clinical applications.