HansH.Ussing教授(1911-2000)是上皮细胞生物学领域的创始成员之一。他最著名的是他开发的用于测量上皮组织中的电离子通量的电生理技术。Usss式电生理学已应用于多种组织,并为两栖动物生物学和医学等领域提供了信息。在后者中,这项技术有助于对高血压等疾病有基本的了解,多囊肾病,囊性纤维化,和腹泻疾病仅举几例。除了对生物学方法的宝贵贡献之外,Prof.在阐明NaKATPase之前的几年,Ussing还为主动运输的概念提供了强有力的证据。此外,他为细胞生物学家提供了极化上皮的重要概念,在根尖和基底外侧膜中发现了特定和不同的转运蛋白,从而为这些细胞提供定向传导的能力,主动和被动跨上皮运输。我的研究使用Ussing室电生理学研究蟾蜍膀胱,两栖动物细胞系,肾细胞系,and,最近,脉络丛细胞系.该技术已成为我们的体外机理研究的基础,该研究以迭代的方式与动物模型一起使用,以更好地了解疾病的进展和治疗。我很荣幸被邀请发表由美国生理学会上皮运输小组赞助的2022年汉斯·乌辛讲座。这份手稿是那次演讲中介绍的材料的一个版本。
Professor Hans H. Ussing (1911-2000) was one of the founding members of the field of epithelial cell biology. He is most famous for the electrophysiological technique that he developed to measure electrogenic ion flux across epithelial tissues. Ussing-style electrophysiology has been applied to multiple tissues and has informed fields as diverse as amphibian biology and medicine. In the latter, this technique has contributed to a basic understanding of maladies such as hypertension, polycystic kidney disease, cystic fibrosis, and diarrheal diseases to mention but a few. In addition to this valuable contribution to biological methods, Prof. Ussing also provided strong evidence for the concept of active transport several years before the elucidation of Na+K+ATPase. In addition, he provided cell biologists with the important concept of polarized epithelia with specific and different transporters found in the apical and basolateral membranes, thus providing these cells with the ability to conduct directional, active and passive transepithelial transport. My studies have used Ussing chamber electrophysiology to study the toad urinary bladder, an amphibian cell line, renal cell lines, and, most recently, choroid plexus cell lines. This technique has formed the basis of our in vitro mechanistic studies that are used in an iterative manner with animal models to better understand disease progress and treatment. I was honored to be invited to deliver the 2022 Hans Ussing Lecture sponsored by the Epithelial Transport Group of the American Physiological Society. This manuscript is a version of the material presented in that lecture.