Abstract：
The gastrointestinal (GI) tract is a series of hollow organs that play roles in food digestion and nutrient absorption. To perform these functions, they should recognize the luminal environment and elicit adequate physiological responses, including digestive juice secretion, peristaltic movements, etc. The Ussing chamber technique is an electrophysiological method for measuring transepithelial ion transport and permeability as short-circuit current (Isc) and transepithelial electrical tissue conductance (Gt) or resistance (TEER), respectively, in vitro. This technique can be applied for the measurement of luminal nutrient sensing and absorption. This article introduces practical methods for measuring luminal nutrient sensing and absorption using intestinal mucosa specimens isolated from humans and experimental animals.
摘要：
胃肠道（GI）是一系列在食物消化和营养吸收中起作用的中空器官。为了执行这些功能，他们应该认识到管腔环境并引起足够的生理反应，包括消化液分泌，蠕动运动,等。Ussing室技术是一种电生理学方法，用于测量跨上皮离子传输和渗透性，例如短路电流（Isc）和跨上皮组织电导（Gt）或电阻（TEER），分别,在体外。该技术可应用于管腔营养传感和吸收的测量。本文介绍了使用从人类和实验动物中分离的肠粘膜标本测量管腔营养传感和吸收的实用方法。
