PDF(Pubmed)
Abstract：
In clinical trials, rhubarb extract (Rb) was demonstrated to efficiently alleviate constipation. We would like to find out the underlying mechanism of rhubarb relieving constipation. However, there are few studies on the effects of rhubarb on colonic mucus secretion and constipation. The aim of this study was to investigate the effects of rhubarb on colonic mucus secretion and its underlying mechanism. The mice were randomly divided into four groups. Group I was the control group and Group II was the rhubarb control group, with Rb (24 g/kg body weight [b.w.]) administered through intragastric administration for three days. Group III mice were given diphenoxylate (20 mg/kg b.w.) for five days via gavage to induce constipation. Group IV received diphenoxylate lasting five days before undergoing Rb administration for three days. The condition of the colon was evaluated using an endoscope. Particularly, the diameter of blood vessels in the colonic mucosa expanded considerably in constipation mice along with diminishing mucus output, which was in line with the observation via scanning electron microscope (SEM) and transmission electron microscope (TEM). We also performed metagenomic analysis to reveal the microbiome related to mucin gene expression level referring to mucin secretion. In conclusion, Rb relieves constipation by rebuilding mucus homeostasis and regulating the microbiome.
摘要：
在临床试验中,大黄提取物（Rb）被证明可以有效缓解便秘。我们想找出大黄缓解便秘的潜在机制。然而,关于大黄对结肠粘液分泌和便秘的影响的研究很少。本研究旨在探讨大黄对结肠黏液分泌的影响及其机制。将小鼠随机分为四组。Ⅰ组为对照组,Ⅱ组为大黄对照组,通过胃内给药给予Rb(24g/kg体重[b.w.])三天。第III组小鼠通过管饲法给予苯乙氧基化物(20mg/kgb.w.)五天以诱导便秘。第IV组接受苯乙氧基化物持续五天，然后进行Rb给药三天。使用内窥镜评价结肠的状况。特别是,便秘小鼠结肠粘膜血管的直径随着粘液输出的减少而大大扩张，这与通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察的结果一致。我们还进行了宏基因组分析，以揭示与粘蛋白分泌相关的粘蛋白基因表达水平的微生物组。总之,Rb通过重建粘液稳态和调节微生物组来缓解便秘。
