这项研究的目的是研究维生素C的生物活性和可持续的生物炼制发展,有机硒,甜菜碱,和石榴皮上的表现,盲肠发酵,和生长兔的抗氧化状态,以减轻热应激的负面影响。将105只35日龄的雄性兔随机分为5组(每组21只),平均体重为752.55±27.56g。实验饮食组包括饲喂不含添加剂的基础饮食的对照组(对照组)和四个治疗组,A,B,C,D,饲喂基础饮食,补充1000毫克维生素C/千克饮食,25mg有机硒/kg饮食,1000毫克甜菜碱/公斤的饮食,或20克石榴皮/公斤饮食,分别。试验中未包括阴性组(非热应激)。兔子给A,B,C,D补充剂的生长性能显着提高(p<0.05),营养消化,总挥发性脂肪酸(VFA)浓度,乙酸,和丙酸,和细菌总数,NH3-N浓度显着降低(p<0.05),肠球菌,大肠杆菌,和大肠杆菌在兔子的盲肠中计数。总蛋白质,白蛋白,球蛋白,超氧化物歧化酶(SOD),过氧化氢酶(CAT),还原型谷胱甘肽(GSH),和谷胱甘肽过氧化物酶(GPx)显著高于(p<0.05)补充A组,B,C,和D补充剂与对照组相比。总胆固醇,甘油三酯,肌酐,尿素,A组丙二醛(MDA)显著降低(p<0.05),B,C,和D补充剂与对照组相比。总之,发现表明补充维生素C,有机硒,甜菜碱,石榴皮在缓解热应激方面发挥了重要作用,对兔的性能有进一步的有益影响,盲肠发酵和微生物群,和抗氧化状态。然而,甜菜碱补充对热应激兔的营养和生理状况具有优越的积极作用。
The aim of this study was to investigate the biological activity and sustainable biorefinery development of vitamin C, organic selenium, betaine, and pomegranate peel on the performance, cecal fermentation, and antioxidant status of growing rabbits to alleviate the negative impacts of heat stress. A total of 105 male rabbits at 35 days old with an average weight of 752.55 ± 27.56 g were randomly assigned to five groups (21 rabbits in each). The experimental dietary groups included a control group fed a basal diet without additives (control group) and four treated groups, A, B, C, and D, fed a basal diet supplemented with either a 1000 mg vitamin C/kg diet, a 25 mg organic selenium/kg diet, a 1000 mg betaine/kg diet, or a 20 g pomegranate peel/kg diet, respectively. No negative group (not heat-stressed) was included in the trial. Rabbits given A, B, C, and D supplements showed a significant increase (p < 0.05) in growth performance, nutrient digestion, concentration of total volatile fatty acids (VFA), acetic, and propionic acids, and total bacterial count, and a significant decrease (p < 0.05) in NH3-N concentration, Enterococcus, coliforms, and E. coli counts in the cecum of rabbits. Total protein, albumin, globulin, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) were significantly (p < 0.05) higher in groups supplemented with A, B, C, and D supplements compared to those in the control group. Total cholesterol, triglycerides, creatinine, urea, and malondialdehyde (MDA) were significantly (p < 0.05) lower in groups supplemented with A, B, C, and D supplements compared to those in the control group. In conclusion, the finding showed that the supplementation of vitamin C, organic selenium, betaine, and pomegranate peel played a significant role in alleviating heat stress and had a further beneficial impact on rabbit performance, cecal fermentation and microbiota, and antioxidant status. However, betaine supplementation had a superior positive effect on the nutritional and physiological profile of heat-stressed rabbits.