PDF(Pubmed)
Abstract：
This is a comparative study to evaluate the effectiveness of six pomegranate peel extracts (PPEs) as antibacterial and antiproliferative agents. The Six PPEs were prepared using four solvent systems and each filtrate was concentrated to a gummy material to be used in the evaluation. The well-diffusion method was used to evaluate their antimicrobial activity against bacteria typically associated with food spoilage: Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, and three Bacillus species. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT) was used to evaluate the cytotoxicity against colorectal carcinoma cells (HCT116), prostate adenocarcinoma (PC3), ovarian cancer cells (SKOV-3), and fibroblasts (MRC-5). The antioxidant evaluation was done using the 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) assay. The pH of the water-containing extracts was acidic and almost the same over 6 weeks. The six PPEs inhibited the bacterial growth in a comparable level to standard antibiotics. The effectiveness of each extract was dependent on the bacterial strain, and the Listeria showed a remarkable inhibition when exposed to the aqueous extract prepared at room temperature (RT). The aqueous (RT) and methanol PPEs had a significant antioxidant scavenging capability and a remarkable cytotoxic activity against the PC3 with half maximal inhibitory concentration (IC50) of 0.1 μg/mL. The boiled aqueous extract exhibited antiproliferative activity against HCT116 with an IC50 of 21.45 μg/mL. The effect on SKOV-3 and fibroblasts was insignificant. With the exception of butanol, the antioxidant screening shows an inverse correlation between the polarity of the extraction solvent and the IC50 exhibited by the PPEs. The variation in the effectiveness of PPEs is suggested to be due to variable soluble bioactive compounds that may interact differently with different cells, though water-containing extracts are promising antibacterial agents. The findings clearly show that pomegranate peel possessed the potential to be an eco-friendly novel source for natural compounds that can be implemented in the food industry as a natural antimicrobial and natural food additive to prevent foodborne illnesses.
摘要：
这是一项比较研究，旨在评估六种石榴皮提取物（PPE）作为抗菌和抗增殖剂的有效性。使用四种溶剂系统制备六种PPE,并将每种滤液浓缩成胶状材料以用于评估。良好扩散方法用于评估它们对通常与食物腐败相关的细菌的抗菌活性：大肠杆菌，铜绿假单胞菌,鼠伤寒沙门氏菌,单核细胞增生李斯特菌,表皮葡萄球菌，金黄色葡萄球菌,和三种芽孢杆菌。3-（4,5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑（MTT）用于评估对结直肠癌细胞的细胞毒性（HCT116），前列腺腺癌（PC3），卵巢癌细胞（SKOV-3），和成纤维细胞(MRC-5)。使用2,2-二苯基-1-吡啶酰肼-水合物(DPPH)测定进行抗氧化剂评估。含水提取物的pH是酸性的并且在6周内几乎相同。六种PPE抑制细菌生长的水平与标准抗生素相当。每种提取物的有效性取决于细菌菌株，当暴露于在室温(RT)下制备的水提取物时,李斯特菌显示出显著的抑制作用。水性（RT）和甲醇PPEs具有显着的抗氧化剂清除能力和对PC3的显着细胞毒性活性，半数最大抑制浓度（IC50）为0.1μg/mL。煮沸的水性提取物对HCT116表现出抗增殖活性，IC50为21.45μg/mL。对SKOV-3和成纤维细胞的影响不显著。除了丁醇,抗氧化剂筛选显示提取溶剂的极性与PPEs显示的IC50之间的负相关。PPEs有效性的变化被认为是由于可变的可溶性生物活性化合物可能与不同的细胞不同地相互作用。虽然含水提取物是有前途的抗菌剂。研究结果清楚地表明，石榴皮具有成为天然化合物的生态友好型新来源的潜力，可以在食品工业中作为天然抗菌和天然食品添加剂来预防食源性疾病。
