烟草黑小腿(TBS),由烟草疫霉引起的,是烟草最有害的疾病之一。有许多研究已经检查了丛枝菌根真菌(AMF)和β-氨基丁酸(BABA)单独诱导抗病性的机制。但AMF和BABA对抗病性的协同作用尚未研究。本研讨考核了BABA施用和AMF接种对烟草对TBS免疫反响的协同感化。结果表明,在叶片上喷施BABA可以提高AMF的定殖率,AMF和BABA处理的烟草感染的疾病指数低于单独的烟草。AMF和BABA对烟草感染烟草的防治效果高于AMF或BABA和烟草。联合应用AMF和BABA显著增长了N的含量,P,和K在叶子和根部,在联合AMF和BABA治疗中比在单独的烟草P.用AMF和BABA处理的植物的干重比单独用烟草P处理的植物的干重高22.3%。与单独的烟草假单胞菌相比,AMF和BABA的联合治疗增加了Pn,Gs,Tr,和根系活动,而单独的烟草菌减少了Ci,H2O2含量,和MDA水平。SOD,POD,CAT,APX,在AMF和BABA联合处理下,Ph活性和表达水平比单独在烟草P中增加。与单独治疗烟草P.nicotianae相比,AMF和BABA的联合使用增加了GSH的积累,脯氨酸,总酚,和类黄酮.因此,与单独施用AMF或BABA相比,联合施用AMF和BABA可以在更大程度上增强烟草植株的TBS抗性。总之,防御相关氨基酸的应用,结合AMF接种,显着促进烟草的免疫反应。我们的发现提供了新的见解,将有助于开发和使用绿色疾病控制剂。
Tobacco black shank (TBS), caused by Phytophthora nicotianae, is one of the most harmful diseases of tobacco. There are many studies have examined the mechanism underlying the induction of disease resistance by arbuscular mycorrhizal fungi (AMF) and β-aminobutyric acid (BABA) alone, but the synergistic effects of AMF and BABA on disease resistance have not yet been studied. This study examined the synergistic effects of BABA application and AMF inoculation on the immune response to TBS in tobacco. The results showed that spraying BABA on leaves could increase the colonization rate of AMF, the disease index of tobacco infected by P.nicotianae treated with AMF and BABA was lower than that of P.nicotianae alone. The control effect of AMF and BABA on tobacco infected by P.nicotianae was higher than that of AMF or BABA and P.nicotianae alone. Joint application of AMF and BABA significantly increased the content of N, P, and K in the leaves and roots, in the joint AMF and BABA treatment than in the sole P. nicotianae treatment. The dry weight of plants treated with AMF and BABA was 22.3% higher than that treated with P.nicotianae alone. In comparison to P. nicotianae alone, the combination treatment with AMF and BABA had increased Pn, Gs, Tr, and root activity, while P. nicotianae alone had reduced Ci, H2O2 content, and MDA levels. SOD, POD, CAT, APX, and Ph activity and expression levels were increased under the combined treatment of AMF and BABA than in P.nicotianae alone. In comparison to the treatment of P.nicotianae alone, the combined use of AMF and BABA increased the accumulation of GSH, proline, total phenols, and flavonoids. Therefore, the joint application of AMF and BABA can enhance the TBS resistance of tobacco plants to a greater degree than the application of either AMF or BABA alone. In summary, the application of defense-related amino acids, combined with inoculation with AMF, significantly promoted immune responses in tobacco. Our findings provide new insights that will aid the development and use of green disease control agents.