PDF(Pubmed)
Abstract：
Banana is one of the most important fruits in the world due to its status as a major food source for more than 400 million people. Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) causes substantial losses of banana crops every year, and molecular host resistance mechanisms are currently unknown. We here performed a genomewide analysis of the autophagy-related protein 8 (ATG8) family in a wild banana species. The banana genome was found to contain 10 MaATG8 genes. Four MaATG8s formed a gene cluster in the distal part of chromosome 4. Phylogenetic analysis of ATG8 families in banana, Arabidopsis thaliana, citrus, rice, and ginger revealed five major phylogenetic clades shared by all of these plant species, demonstrating evolutionary conservation of the MaATG8 families. The transcriptomic analysis of plants infected with Foc TR4 showed that nine of the MaATG8 genes were more highly induced in resistant cultivars than in susceptible cultivars. Finally, MaATG8F was found to interact with MaATG4B in vitro (with yeast two-hybrid assays), and MaATG8F and MaATG4B all positively regulated banana resistance to Foc TR4. Our study provides novel insights into the structure, distribution, evolution, and expression of the MaATG8 family in bananas. Furthermore, the discovery of interactions between MaATG8F and MaATG4B could facilitate future research of disease resistance genes for the genetic improvement of bananas.
摘要：
香蕉是世界上最重要的水果之一，因为它是超过4亿人的主要食物来源。尖孢镰刀菌f.sp.古巴热带种族4（FocTR4）每年都会造成香蕉作物的大量损失，和分子宿主抗性机制目前尚不清楚。我们在这里对野生香蕉物种中的自噬相关蛋白8（ATG8）家族进行了全基因组分析。发现香蕉基因组包含10个MaATG8基因。四个MaATG8s在4号染色体远端构成了一个基因簇。香蕉ATG8家族的系统发育分析,拟南芥，柑橘,大米,生姜揭示了所有这些植物物种共有的五个主要的系统发育分支，证明了MaATG8家族的进化保守性。感染FocTR4的植物的转录组学分析表明，抗性品种中的9个MaATG8基因的诱导率高于易感品种。最后,发现MaATG8F在体外与MaATG4B相互作用（使用酵母双杂交测定），MaATG8F和MaATG4B均正调节香蕉对FocTR4的抗性。我们的研究提供了对结构的新颖见解，分布，进化,以及MaATG8家族在香蕉中的表达。此外，MaATG8F和MaATG4B之间相互作用的发现可以促进未来抗病基因的研究，以改善香蕉的遗传。
