对苦参素叶中甲醇提取物的化学研究导致分离出一种新的酚类葡萄糖苷(1)以及15种已知的次生代谢产物,即山芝苷甲酯(2),月桂酰胺乙酸酯(3),咖啡酸甲酯(4),咖啡酸(5),芹菜素(6),木犀草素(7),山奈酚(8),槲皮素(9),亚甲酸(10),木犀草素-7-O-β-D-吡喃葡萄糖苷(11),木犀草素-4'-O-β-D-吡喃葡萄糖苷(12),山奈酚-7-O-α-L-鼠李糖苷(13),山奈酚-3-O-β-D-吡喃葡萄糖基-(1→6)-O-α-L-鼠李糖苷(14),山奈酚-3,7-O-α-L-二鼠李糖苷(15)和(2S,3S,4R,8E)-2-((2'R)-2'-羟基-十八烷酰氨基)-木素二烷-1,3,4-三醇-8-烯(16)。通过1D和2DNMR分析和质谱阐明了它们的结构。评价提取物和分离的化合物的抗菌活性。EtOAc提取物对大肠杆菌和粪肠杆菌具有高活性(MIC=32和64μg/mL),分别。化合物1、2、2b、5、8、9和12(MIC=16-32μg/mL)对一些测试细菌具有中等活性。
Chemical study of the methanol extract from the leaves of Flacourtia flavescens led to the isolation of a new phenolic glucoside (1) along with fifteen known secondary metabolites namely shanzhiside methyl ester (2), aurantiamide acetate (3), caffeic acid methyl ester (4), caffeic acid (5), apigenin (6), luteolin (7), kaempferol (8), quercetin (9), gyrophoric acid (10), luteolin-7-O-β-D-glucopyranoside (11), luteolin-4\'-O-β-D-glucopyranoside (12), kaempferol-7-O-α-L-rhamnopyranoside (13), kaempferol-3-O-β-D-glucopyranosyl-(1→6)-O-α-L-rhamnopyranoside (14), kaempferol-3,7-O-α-L-dirhamnopyranoside (15) and (2S,3S,4R,8E)-2-((2\'R)-2\'-hydroxy-octadecanoylamino)-lignocerane-1,3,4-triol-8-ene (16). Their structures were elucidated by 1D and 2D NMR analysis and mass spectrometry. The extracts and the isolated compounds were evaluated for their antibacterial activities. The EtOAc extract was highly active (MIC = 32 and 64 µg/mL) against E. coli and E. faecalis, respectively. Compounds 1, 2, 2b, 5, 8, 9, and 12 (MIC = 16-32 µg/mL) were moderately active against some tested bacteria.