目的:本研究旨在评估两种最新一代的基质辅助激光解吸电离-飞行时间质谱(MALDI-TOFMS)系统在常规实验室设置中的性能,专注于周转时间(TAT),到达结果的时间(TTR),动手时间,和识别率。
方法:我们对三种工作流程场景进行了时间和运动研究,以模拟不同的实验室设置。总的来说,使用VITEKMSPRIME(bioMérieux)和MALDIBiotypersirius(BrukerDaltonics)及其相应的数据库VITEKIVD数据库3.2和MBT参考库12处理了来自三级医院实验室的618种细菌分离株。
结果:目标制备过程显示TAT没有显着差异,但是Biotyper工作流程的动手时间缩短了3至6分钟。在测量过程中,Biotyper天狼星的TTR缩短了三到五倍,而VITEKMSPRIME的动手时间则明显缩短(每个目标约1.5分钟)。VITEKMSPRIME的识别率为97.9%,Biotypersirius的识别率为98.9%。两个系统在属上均达到100%的一致性,在物种水平上达到96.2%的一致性。
结论:两种系统对常规细菌分离株都表现出优异的识别率。由于速度快,Biotypersirius适用于具有高样品通量和设计用于处理较大批次的工作流程的实验室。VITEKMSPRIME,其“加载和运行”系统可容纳多达16个目标,减少动手时间,使其成为一个合理的选择,实验室具有较少的识别整体,但更多的目标和工作流设计的并行处理在不同的工作站。
OBJECTIVE: This study aims to evaluate the performance of two latest generation matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems in routine laboratory settings, focusing on turnaround time (TAT), time to results (TTR), hands-on time, and identification rate.
METHODS: We conducted a time and motion study on three workflow scenarios to simulate different laboratory settings. Overall, 618 bacterial isolates from a tertiary hospital\'s laboratory were processed using the VITEK MS PRIME (bioMérieux) and the MALDI Biotyper sirius (Bruker Daltonics) and their corresponding databases VITEK IVD Database 3.2 and MBT reference library 12.
RESULTS: The target preparation process showed no significant difference in TAT, but the Biotyper workflow had a shorter hands-on time by 3 to 6 min. In the measurement process, TTR was three to five times shorter for the Biotyper sirius while hands-on time was significantly shorter for VITEK MS PRIME (approximately 1.5 min per target). The identification rate without retesting was 97.9% for VITEK MS PRIME and 98.9% for Biotyper sirius. Both systems achieved 100% agreement at genus and 96.2% at species level.
CONCLUSIONS: Both systems exhibited excellent identification rates for routine bacterial isolates. Due to its high speed, the Biotyper sirius is suited for laboratories with high sample throughput and a workflow designed for processing larger batches. The VITEK MS PRIME, with its \"load and go\" system accommodating up to 16 targets, reduces hands-on time, making it a reasonable choice for laboratories with fewer identifications overall but a higher number of targets and a workflow designed for parallel processing on different workstations.