Abstract：
Burkholderia pseudomallei is a Gram-negative saprophytic bacillus that causes melioidosis. The infection is endemic in South-East of Asia and Northern Australia. B. pseudomallei has been designated as bioterrorism agent and its manipulation should be done in a biological safety level 3 capability. Workers in laboratories may be accidentally exposed to B. pseudomallei before its identification, with a risk of laboratory-acquired melioidosis. We want to describe a case of melioidosis occurred in our hospital and its management at laboratory. The objective of this article is to provide guidance to microbiologists confronted with a suspicious case of B. pseudomallei on the management of the exposition. We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.
摘要：
假伯克霍尔德菌是一种革兰氏阴性腐生杆菌，可引起类骨病。这种感染在亚洲东南部和澳大利亚北部流行。假单胞菌已被指定为生物恐怖主义剂，其操作应在生物安全3级能力下进行。实验室的工作人员在鉴定前可能会意外接触到假大肠杆菌，有实验室获得性类骨病的风险。我们想描述一例在我们医院发生的类骨病及其在实验室的管理。本文的目的是为面对可疑的B.pseudomallei病例的微生物学家提供有关博览会管理的指导。我们在这里报告了一些微生物学论据，这些论据通常可以指导微生物学家进行假单胞菌的推定鉴定。此病例报告显示了MALDI-TOFMS准确数据库的重要性，以确保准确的微生物识别和适应暴露个体的抗生素预防。我们还想强调，必须制定有效的战略，预防微生物实验室可能发生的任何意外接触。
