目的:新生儿坏死性小肠结肠炎(NEC)是一种严重的肠道炎症性疾病。我们研究了肠道脂肪酸结合蛋白(I-FABP),I-FABPmRNA,和白细胞介素-6(IL-6)作为NEC的潜在诊断生物标志物。
方法:对40只小鼠进行缺氧缺血肠损伤,然后对血清I-FABP蛋白和mRNA水平进行定量。回肠组织病理评分采用苏木精、伊红染色。使用蛋白质印迹和免疫荧光检测这些组织中的I-FABP表达水平和易位,分别。来自30名患有NEC的人新生儿和30名健康新生儿的样品测量了血清I-FABP蛋白/mRNA和IL-6水平。
结果:小鼠回肠组织病理评分和I-FABP水平,以及血清I-FABP和I-FABPmRNA水平,模型组明显高于对照组。血清I-FABP,I-FABPmRNA,NEC新生儿的IL-6水平明显高于健康组。Logistic回归和受试者工作曲线分析显示,I-FABP蛋白/mRNA和IL-6水平可能是NEC的诊断生物标志物。
结论:I-FABP蛋白/mRNA和IL-6水平是NEC新生儿肠缺血性损伤的有用生物标志物。建议联合检测I-FABP蛋白/mRNA和IL-6,而不是使用单一的生物标志物。
OBJECTIVE: Neonatal necrotizing enterocolitis (NEC) is a serious intestinal inflammatory disease. We investigated intestinal fatty acid binding protein (I-FABP), I-FABP mRNA, and interleukin-6 (IL-6) as potential diagnostic biomarkers in NEC.
METHODS: Forty mice were subjected to hypoxic-ischemic intestinal injury, and then serum I-FABP protein and mRNA levels were quantified. Ileal tissue pathological scores were determined by hematoxylin and eosin staining. I-FABP expression levels and translocation in these tissues were detected using western blotting and immunofluorescence, respectively. Samples from 30 human neonates with NEC and 30 healthy neonates had serum I-FABP protein/mRNA and IL-6 levels measured.
RESULTS: The mouse ileal tissue pathological score and I-FABP levels, as well as serum I-FABP and I-FABP mRNA levels, were significantly higher in the model group than in the control group. Serum I-FABP, I-FABP mRNA, and IL-6 levels were significantly higher in human neonates with NEC than in the healthy group. Logistic regression and receiver operating curve analyses revealed that I-FABP protein/mRNA and IL-6 levels could be diagnostic biomarkers for NEC.
CONCLUSIONS: I-FABP protein/mRNA and IL-6 levels are useful biomarkers of intestinal ischemic injury in neonates with NEC. The combined detection of I-FABP protein/mRNA and IL-6 is recommended rather than using a single biomarker.