压力对精神和消化系统健康的影响已被广泛研究,慢性压力与各种疾病有关。然而,对急性应激反应的年龄相关差异,行为和生理上,仍然知之甚少。因此,本研究旨在建立一个模型来检测不同年龄小鼠的瞬时应激。我们实验中使用的压力源是约束压力程序,对小鼠进行短暂的固定以诱导急性应激反应。将3、6、12和30周龄的雄性C3H/HeN小鼠置于50ml锥形离心管中15分钟,经受急性克制应激(ARS)。随后,他们的行为,器官组织,血液学参数,皮质醇浓度,和免疫反应进行了评估。在ARS之后,压力后12周龄小鼠的时间和进入中心的时间增加。与其他年龄的老鼠相比,那些6周大的人表现出红细胞明显升高,血小板,血红蛋白,和血细胞比容,所有这些都受到时间依赖性变化和ARS恢复过程的影响。ARS后所有年龄组的血液皮质酮水平均显着升高。此外,ARS诱导白细胞显著增加,嗜碱性粒细胞,住宅巨噬细胞,除3周龄小鼠外,所有年龄组的CD4+T细胞。然而,单核细胞来源的巨噬细胞和CD8+T细胞的数量没有显著变化.此外,3和6周龄的小鼠在ARS后显示GFAP+细胞增加,而NeuN+细胞在所有年龄段都减少。这些结果表明ARS对行为有不同的影响,皮质醇浓度,不同年龄小鼠的血细胞和肝脏免疫细胞的数量。这些与年龄有关的反应揭示了压力和生理系统之间复杂的相互作用,并有助于对压力相关疾病的更广泛理解。
The impact of stress on mental and digestive health has been extensively studied, with chronic stress being associated with various disorders. However, age-related differences in the response to acute stress, both behaviorally and physiologically, remain poorly understood. Therefore, this study aimed to develop a model to detect transient stress in mice of different ages. The stressor employed in our experiments was a restraint stress procedure, where mice were subjected to brief periods of immobilization to induce an acute stress response. Male C3H/HeN mice aged 3, 6, 12, and 30 weeks were subjected to acute restrain stress (ARS) by being placed in a 50 ml conical centrifuge tube for 15 min. Subsequently, their behavior, organ tissues, hematological parameters, cortisol concentration, and immune responses were assessed. Following ARS, the increased in time and entries into the center by the 12-week-old mice following stress. In comparison to mice of other ages, those aged 6 weeks demonstrated notable elevations in erythrocytes, platelets, hemoglobin, and hematocrit, all of which were influenced by the time-dependent changes and the recovery process of ARS. Blood corticosterone levels were substantially elevated in all age groups after ARS. Furthermore, ARS induced a notable increase in leukocytes, basophils, residential macrophages, and CD4+ T cells in all age groups except for 3-week-old mice. However, the number of monocyte-derived macrophages and CD8+ T cells did not change significantly. Additionally, mice aged 3 and 6 weeks demonstrated an increase in GFAP+ cells following ARS, whereas NeuN+ cells decreased across all ages. These results suggest that ARS has varying effects on the behavior, cortisol concentration, and quantity of blood cells as well as hepatic immune cells in mice of different ages. These age-dependent responses shed light on the complex interplay between stress and physiological systems and contribute to the broader understanding of stress-related diseases.