化疗药物,如阿霉素(Dox),通常用于治疗各种恶性肿瘤。然而,Dox诱导的心脏毒性限制了药物的临床应用。因此,这项研究旨在研究地奥司明是否可以预防或限制Dox在动物环境中引起的心脏毒性。将32只大鼠分为四个不同的对照组,那些用Dox(20毫克/千克,腹膜内,i.p.),那些用地奥司明100毫克加Dox治疗的,和那些用地奥司明200毫克加Dox治疗。实验结束时,将大鼠麻醉并处死,收集它们的血液和心脏。对血液中的心脏毒性标志物进行了分析,心脏组织通过生化检测MDA进行分析,GSH,CAT,蛋白质印迹分析(NF-kB,IL-6,TLR-4,TNF-α,iNOS,和COX-2),和基因表达分析(β-MHC,BNP)。福尔马林固定的组织用于组织病理学研究。我们证明了Dox损伤会导致氧化应激增加,炎症,和肥大,如MDA水平增加,GSH含量和CAT活性降低。此外,Dox治疗引起心脏肥大和损伤,生化分析证明,ELISA,蛋白质印迹分析,和基因表达分析。然而,两种剂量的地奥司明共同给药,100毫克和200毫克,减轻了这些改变。来自当前研究的数据表明,地奥司明的心脏保护作用可能是由于其减轻氧化应激和炎症的能力。然而,需要进一步的研究来研究地奥司明对Dox诱导的心脏毒性的保护作用。
Chemotherapeutic drugs, such as doxorubicin (Dox), are commonly used to treat a variety of malignancies. However, Dox-induced cardiotoxicity limits the drug\'s clinical applications. Hence, this study intended to investigate whether
diosmin could prevent or limit Dox-induced cardiotoxicity in an animal setting. Thirty-two rats were separated into four distinct groups of controls, those treated with Dox (20 mg/kg, intraperitoneal, i.p.), those treated with diosmin 100 mg plus Dox, and those treated with
diosmin 200 mg plus Dox. At the end of the experiment, rats were anesthetized and sacrificed and their blood and hearts were collected. Cardiac toxicity markers were analyzed in the blood, and the heart tissue was analyzed by the biochemical assays MDA, GSH, and CAT, western blot analysis (NF-kB, IL-6, TLR-4, TNF-α, iNOS, and COX-2), and gene expression analysis (β-MHC, BNP). Formalin-fixed tissue was used for histopathological studies. We demonstrated that a Dox insult resulted in increased oxidative stress, inflammation, and hypertrophy as shown by increased MDA levels and reduced GSH content and CAT activity. Furthermore, Dox treatment induced cardiac hypertrophy and damage, as evidenced by the biochemical analysis, ELISA, western blot analysis, and gene expression analysis. However, co-administration of
diosmin at both doses, 100 mg and 200 mg, mitigated these alterations. Data derived from the current research revealed that the cardioprotective effect of
diosmin was likely due to its ability to mitigate oxidative stress and inflammation. However, further study is required to investigate the protective effects of
diosmin against Dox-induced cardiotoxicity.