Abstract：
Doxorubicin (DOX) can be applied to treat several cancers. DOX-induced oxidative stress causes testicular damage. Diosmin (DIO), as a potent antioxidant, reduces many drugs\' side effects. We determined DIO therapeutic effects on DOX-related testicular toxicity. Forty rats were assigned to five groups as control, DOX (2.5 mg/kg six i.p. injections at equal intervals over two weeks), DOX + DIO (25, 50, 100 mg/kg, orally, daily, for two weeks) groups. Oxidative and antioxidant markers, fertility parameters levels, sperm parameters, and a histopathological examination were analyzed. DOX group showed a significant decrease in the number of spermatogonia, primary spermatocytes, and sertoli cells, seminiferous tubular diameter, seminiferous luminal diameter, and seminiferous epithelial height. Moreover, testosterone levels, glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities showed a significant decrease. Furthermore, nitric oxide (NO) and malondialdehyde (MDA) contents and also follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels showed a significant increase in the DOX group compared to the control group. DIO improved DOX-related alterations in levels of hormones, spermatogonia, spermatocytes, and sertoli cell number, and seminiferous diameters (tubular, luminal, and epithelial height). Furthermore, GSH level, SOD, GPx, and CAT activities showed a significant increase, and MDA and NO contents showed a significant decrease in the DOX + DIO group than the DOX group. The results indicate that DIO mitigate DOX-induced testicular toxicity by its anti-oxidant activity.
摘要：
阿霉素(DOX)可用于治疗多种癌症。DOX诱导的氧化应激导致睾丸损伤。Diosmin(DIO),作为一种有效的抗氧化剂，减少许多药物的副作用。我们确定了DIO对DOX相关睾丸毒性的治疗作用。将40只大鼠分为5组作为对照,DOX（2.5mg/kg，在两周内以相等的间隔进行6次腹膜内注射），DOX+DIO（25、50、100mg/kg，口头,daily,两周)组。氧化和抗氧化标记，生育率参数水平，精子参数,并对组织病理学检查进行了分析。DOX组精原细胞数量显著减少，原代精母细胞，和支持细胞，生精管直径，生精腔直径，和生精上皮高度。此外，睾酮水平,谷胱甘肽（GSH）水平，过氧化氢酶(CAT),谷胱甘肽过氧化物酶（GPx），超氧化物歧化酶(SOD)活性显著下降。此外，与对照组相比，DOX组的一氧化氮（NO）和丙二醛（MDA）含量以及卵泡刺激素（FSH）和黄体生成素（LH）水平显着增加。DIO改善了与DOX相关的激素水平变化，精原细胞,精母细胞,和支持细胞数，和生精直径(管状，管腔,和上皮高度）。此外，GSH水平,SOD,GPx,CAT活动显着增加，而MDA和NO含量在DOX+DIO组较DOX组显著降低。结果表明，DIO通过其抗氧化活性减轻DOX诱导的睾丸毒性。
