这项研究调查了遗传多样性对花生变应原性的影响,并使用Balb/c小鼠模型评估了六个花生品种的变应原能力。它还使用计算工具探索了Arah3(花生过敏原)和Glym(大豆过敏原)之间的潜在交叉反应性。向雌性Balb/c小鼠注射花生蛋白提取物和明矾。血清特异性抗体(IgE,IgGt,使用ELISA测量IgG1,IgG2a),和过敏性蛋白质谱通过蛋白质印迹检查。结构同源性,B细胞表位,并研究了Arah3和Glym与人IgE的分子相互作用。小鼠产生了高sIgE和sIgG1反应,抗体识别蛋白质印迹上的19条条带。值得注意的是,撒哈拉种质显示出独特的特征,例如蛋白质印迹图谱上没有条带,减轻过敏症状,较低的IgE滴度,和较少的肠组织损伤。分子对接结果表明显著的交叉致敏性,由变应原性预测和结构同源性分析支持。这种全面的分析提供了对共享表位的见解,结合位点的潜在竞争,和交叉反应反应的分子动力学,加强对食物过敏原相互作用的理解。该研究建议在育种中使用阿尔及利亚撒哈拉花生种质,基因组学研究,和行业为过敏个体提供更安全的花生选择。意义:这项研究的意义在于它对解决一个主要的公共卫生问题:花生过敏,这是影响全球许多个人和家庭的过敏反应的重要原因。通过实验和计算方法探索花生蛋白的遗传多样性并鉴定低变应原性种质,这项研究为减轻过敏反应提供了有价值的见解。研究结果突出表明,来自撒哈拉地区的某些种质表现出降低的致敏性,导致过敏性症状减弱,较低的IgE水平,并减少小鼠模型的肠道损伤。此外,这项研究的计算机模拟分析揭示了花生和大豆过敏原之间的交叉反应性问题,为在分子水平上理解过敏原相互作用提供重要信息。总的来说,这项研究有助于提高食品过敏原研究领域的知识,并对提高对花生过敏的个体的生活质量具有实际意义,特别是通过选择更安全的花生品种及其种植。
This study investigated the effects of genetic diversity in the allergenicity of peanut and assessed the allergenic capacity of six Arachis hypogaea accessions using a Balb/c mouse model. It also explored potential cross-reactivities between Ara h 3 (peanut allergen) and Gly m (soybean allergen) using computational tools. Female Balb/c mice were injected with peanut protein extracts and alum. Serum-specific antibodies (IgE, IgGt, IgG1, IgG2a) were measured using ELISA, and allergic protein profiles were examined via western blot. Structural homology, B cell epitopes, and molecular interactions between Ara h 3 and Gly m with human IgE were also investigated. The mice developed high sIgE and sIgG1 responses, with antibodies recognizing 19 bands on western blot. Notably, Saharan accessions showed unique features such as no bands on western blot profiles, reduced anaphylactic symptoms, lower IgE titers, and less intestinal tissue damage. Molecular docking results suggest significant cross-allergenicity, supported by allergenicity predictions and structural homology analysis. This comprehensive analysis provides insights into shared epitopes, potential competition for binding sites, and molecular dynamics of cross-reactive responses, enhancing understanding of food allergen interactions. The study recommends using Algerian Sahara peanut accessions in breeding, genomics studies, and industry for safer peanut options for individuals with allergies. SIGNIFICANCE: The significance of this study lies in its contribution to addressing a major public health issue: peanut allergy, which represents a significant cause of anaphylaxis affecting numerous individuals and families worldwide. By exploring the genetic diversity of peanut proteins and identifying hypoallergenic accessions through experimental and computational approaches, this research offers valuable insights for mitigating allergic reactions. The findings highlight that certain accessions from the Saharan region exhibit reduced allergenicity, resulting in attenuated anaphylactic symptoms, lower IgE levels, and reduced intestinal damage in murine models. Furthermore, the study\'s in silico analysis sheds light on the issue of cross-reactivity between peanut and soybean allergens, providing crucial information for understanding allergen interactions at the molecular level. Overall, this research contributes to advancing knowledge in the field of food allergen research and has practical implications for improving the quality of life for individuals allergic to peanuts, particularly through the selection of safer peanut varieties and their cultivation.