■循环Loaloa抗原通常在患有严重的L.loa感染的个体中通过诊断测试检测到由Wuchereriabancrofti引起的淋巴丝虫病(LF)。这对Loiasis共同流行地区的LF制图和消除工作是一个重大挑战。然而,它也提供了一个机会,以确定抗原生物标志物的loiasis。为了确定哪些L.loa抗原可能是有希望的生物标志物,用于区分真LF和loiasis,我们对生活在喀麦隆奥科拉卫生区的一组患有严重L.loa感染的个体进行了L.loa抗原筛查。在这项纵向研究中,通过丝虫病试纸条(FTS)测试参与者的交叉反应性抗原血症,ELISA,和westernblot,并在纳入后6,9,12和15个月监测FTS状态.然后,我们通过液相色谱-串联质谱(LC-MS/MS)从基线和15个月的血浆样品鉴定了特定的循环L.loa抗原。
■在73名FTS阳性(FTS+)和13名FTS阴性(FTS-)参与者中,83%的人在研究过程中保持了FTS状态,而17%的人经历了至少一次FTS转换事件(从FTS+到FTS-或反之亦然)。通过蛋白质印迹在FTS+和FTS-血清中检测到交叉反应抗原,FTS在抗原检测中的一致性很差,westernblot,和ELISA方法。一个蛋白质家族,一组Nas-14金属蛋白酶,在>80%的测试样品中通过LCMS/MS检测到,包括FTS样本。这些数据确定Nas-14是一种有前途的loiasis生物标志物,可能能够区分loiasis和淋巴丝虫病。
UNASSIGNED: Circulating Loa loa antigens are often detected in individuals with heavy L. loa infections by diagnostic tests for lymphatic filariasis (LF) caused by Wuchereria bancrofti. This is a major challenge to LF mapping and elimination efforts in loiasis co-endemic areas. However, it also provides an opportunity to identify antigen biomarkers for loiasis. To determine which L. loa antigens might be promising biomarkers for distinguishing true LF from loiasis, we screened for L. loa antigens in a group of individuals with heavy L. loa infections living in the Okola Health District of Cameroon. In this longitudinal study, participants were tested for cross-reactive antigenemia by filariasis test strip (FTS), ELISA, and western blot, and were monitored for FTS status at 6, 9, 12, and 15 months post-enrollment. We then identified specific circulating L. loa antigens by liquid chromatography-tandem mass spectrometry (LC-MS/MS) from baseline and 15-month plasma samples.
UNASSIGNED: Among 73 FTS-positive (FTS+) and 13 FTS-negative (FTS-) participants with high L. loa microfilarial loads, 83% maintained their FTS status over the course of the study, while 17% experienced at least one FTS conversion event (from FTS+ to FTS- or vice versa). Cross-reactive antigens were detected in both FTS+ and FTS- sera by western blot, and there was poor agreement in antigen detection by FTS, western blot, and ELISA methods. One protein family, a group of Nas-14 metalloproteases, was detected by LC MS/MS in >80% of tested samples, including FTS- samples. These data identify Nas-14 as a promising loiasis biomarker potentially capable of distinguishing loiasis from lymphatic filariasis.