Abstract：
Agar gel immunodiffusion assay (AGID) is a laboratory test which detects specific antigen-antibody interactions by the development of visible precipitation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results. This test has known cross-reactivity to bluetongue antibodies; therefore positive samples by this assay require additional confirmatory testing; generally, its use should be limited to healthy animal attestations where required.
摘要：
琼脂凝胶免疫扩散测定（AGID）是一种实验室测试，通过在半固体基质中形成可见的沉淀线来检测特定的抗原-抗体相互作用。在这里，我们描述琼脂凝胶板的制备，通过AGID测试血清样品中是否存在EHDV抗体的方法，以及测试结果的解释。该测试已知与蓝舌病抗体具有交叉反应性；因此，该测定的阳性样品需要额外的确认测试；通常，它的使用应限于健康的动物证明。
