实现快速,成本有效,黄酮类化合物的智能识别和定量具有挑战性。为了快速简单的类黄酮测定,开发了智能手机耦合比色传感器阵列(电子鼻)的传感平台,依靠橙皮苷的差异化竞争抑制作用,景天苷,和橘皮素对纳米酶的氧化反应具有3,3',5,5'-四甲基联苯胺底物。首先,密度泛函理论计算预测了掺杂Mn后CeO2纳米酶的过氧化物酶样活性增强,Co,Fe,然后通过实验证实了这一点。自行设计的移动应用程序,快速查看器,能够快速评估红色,绿色,和蓝色值的比色图像使用多孔并行采集策略。基于CeMn三通道的传感器阵列,CeFe,CeCo能够区分不同类别的黄酮类化合物,浓度,混合物,通过线性判别分析,研究了富含类黄酮的陈皮的各种储存时间。此外,“分割-提取-回归”深度学习算法的集成使单孔图像能够通过从3×4传感阵列中分割来获得,以增强阵列图像的特征信息。MobileNetV3小型神经网络在37,488个单孔图像上进行了训练,并实现了对类黄酮浓度的出色预测能力(R2=0.97)。最后,MobileNetV3-small作为应用程序(智能分析大师)集成到智能手机中,实现三种浓度的一键输出。这项研究为黄酮类化合物的定性和同时多成分定量分析开发了一种创新方法。
Achieving rapid, cost effective, and intelligent identification and quantification of flavonoids is challenging. For fast and uncomplicated flavonoid determination, a sensing platform of smartphone-coupled colorimetric sensor arrays (electronic noses) was developed, relying on the differential competitive inhibition of hesperidin, nobiletin, and tangeretin on the oxidation reactions of nanozymes with a 3,3\',5,5\'-tetramethylbenzidine substrate. First, density functional theory calculations predicted the enhanced peroxidase-like activities of CeO2 nanozymes after doping with Mn, Co, and Fe, which was then confirmed by experiments. The self-designed mobile application, Quick Viewer, enabled a rapid evaluation of the red, green, and blue values of colorimetric images using a multi-hole parallel acquisition strategy. The sensor array based on three channels of CeMn, CeFe, and CeCo was able to discriminate between different flavonoids from various categories, concentrations, mixtures, and the various storage durations of flavonoid-rich Citri Reticulatae Pericarpium through a linear discriminant analysis. Furthermore, the integration of a \"segmentation-extraction-regression\" deep learning algorithm enabled single-hole images to be obtained by segmenting from a 3 × 4 sensing array to augment the featured information of array images. The MobileNetV3-small neural network was trained on 37,488 single-well images and achieved an excellent predictive capability for flavonoid concentrations (R2 = 0.97). Finally, MobileNetV3-small was integrated into a smartphone as an application (Intelligent Analysis Master), to achieve the one-click output of three concentrations. This study developed an innovative approach for the qualitative and simultaneous multi-ingredient quantitative analysis of flavonoids.