%0 Journal Article
%T Inhibition effect of p-d orbital hybridized PtSn nanozymes for colorimetric sensor array of antioxidants.
%A Jia X
%A Jiao L
%A Li R
%A Yan D
%A Hu L
%A Chen C
%A Li X
%A Zhai Y
%A Lu X
%J Biosens Bioelectron
%V 261
%N 0
%D 2024 Oct 1
%M 38852326
%F 12.545
%R 10.1016/j.bios.2024.116468
%X Rational design of peroxidase (POD)-like nanozymes with high activity and specificity still faces a great challenge. Besides, the investigations of nanozymes inhibitors commonly focus on inhibition efficiency, the interaction between nanozymes-involved catalytic reactions and inhibitors is rarely reported. In this work, we design a p-block metal Sn-doped Pt (p-d/PtSn) nanozymes with the selective enhancement of POD-like activity. The p-d orbital hybridization interaction between Pt and Sn can effectively optimize the electronic structure of PtSn nanozymes and thus selectively enhance POD-like activity. In addition, the antioxidants as nanozymes inhibitors can effectively inhibit the POD-like activity of p-d/PtSn nanozymes, which results in the fact that antioxidants absorbed on the p-d/PtSn surface can hinder the adsorption of hydrogen peroxide. The inhibition type (glutathione as a model molecule) is reversible mixed-inhibition with inhibition constants (Ki' and Ki) of 0.21 mM and 0.03 mM. Finally, based on the varying inhibition levels of antioxidant molecules, a colorimetric sensor array is constructed to distinguish and simultaneously detect five antioxidants. This work is expected to design highly active and specific nanozymes through p-d orbital hybrid engineering, and also provides insights into the interaction between nanozymes and inhibitors.