Abstract：
The integration of smartphones with conventional analytical approaches plays a crucial role in enhancing on-site detection platforms for point-of-care testing. Here, we developed a simple, rapid, and efficient three-channel colorimetric sensor array, leveraging the peroxidase (POD)-like activity of polydopamine-decorated FeNi foam (PDFeNi foam), to identify antioxidants using both microplate readers and smartphones for signal readouts. The exceptional catalytic capacity of PDFeNi foam enabled the quick catalytic oxidation of three typical peroxidase substrates (TMB, OPD and 4-AT) within 3 min. Consequently, we constructed a colorimetric sensor array with cross-reactive responses, which was successfully applied to differentiate five antioxidants (i.e., glycine (GLY), glutathione (GSH), citric acid (CA), ascorbic acid (AA), and tannic acid (TAN)) within the concentration range of 0.1-10 μM, quantitatively analyze individual antioxidants (with AA and CA as model analytes), and assess binary mixtures of AA and GSH. The practical application was further validated by discriminating antioxidants in serum samples with a smartphone for signal readout. In addition, since pesticides could be absorbed on the surface of PDFeNi foam through π-π stacking and hydrogen bonding, the active sites were differentially masked, leading to featured modulation on POD-like activity of PDFeNi foam, thereby forming the basis for pesticides discrimination on the sensor array. The nanozyme-based sensor array provides a simple, rapid, visual and high-throughput strategy for precise identification of various analytes with a versatile platform, highlighting its potential application in point-care-of diagnostic, food safety and environmental surveillance.
摘要：
智能手机与传统分析方法的集成在增强现场检测平台以进行即时检测方面发挥着至关重要的作用。这里,我们开发了一个简单的，快速,和高效的三通道比色传感器阵列，利用聚多巴胺修饰的FeNi泡沫（PDFeNi泡沫）的过氧化物酶（POD）样活性，使用酶标仪和智能手机进行信号读出来识别抗氧化剂。PDFeNi泡沫的特殊催化能力使三种典型的过氧化物酶底物（TMB，OPD和4-AT）在3分钟内。因此,我们构建了一个具有交叉反应响应的比色传感器阵列，它被成功地应用于区分五种抗氧化剂(即，甘氨酸(GLY),谷胱甘肽(GSH),柠檬酸(CA),抗坏血酸(AA),和单宁酸（TAN））在0.1-10μM的浓度范围内，定量分析单个抗氧化剂(以AA和CA作为模型分析物)，并评估AA和GSH的二元混合物。通过用智能手机进行信号读出区分血清样品中的抗氧化剂，进一步验证了实际应用。此外,由于农药可以通过π-π堆积和氢键作用吸附在PDFeNi泡沫表面，活性位点被差异掩盖，导致PDFeNi泡沫的POD样活性的特征调制，从而在传感器阵列上形成农药辨别的基础。基于纳米酶的传感器阵列提供了一种简单的，快速,视觉和高通量策略，用于使用通用平台精确识别各种分析物，强调其在诊断点护理中的潜在应用，食品安全和环境监测。
