块状皮肤病(LSD),羊痘和羊痘是反刍动物的危险疾病。羊痘病毒属的代表在抗原上相似,可以用作三种感染的疫苗,就像正痘病毒属的代表一样,其中包括天花病毒,猴痘,和牛痘,都属于一个单一的痘病毒科。
在这项研究中,使用羊痘病毒疫苗株G20-LKV和LSD病毒强毒株RIBSP2019/K.实验是在哈萨克白头品种的临床健康牛身上进行的,六至八个月大。工作中使用了病毒学和血清学研究方法。
所有接受不同剂量疫苗的免疫动物都表现出对感染攻击的抵抗力,没有任何临床症状.在接受最低剂量疫苗15,000,30,000和40,000TCID50的动物中,没有不良事件,在注射部位观察到皮肤和温度反应.那些接种了高剂量疫苗的人在疫苗施用部位具有肿胀形式的局部反应。感染了毒性病毒的对照动物显示出牛块状皮肤病的临床症状。
疫苗,根据山羊病毒的“G20-LKV”株制备,在15,000至80,000TCID50的免疫剂量下,可以保护牛免受毒性LSD病毒感染,这取决于特定地区的LSD流行情况。
Lumpy skin disease (LSD), sheep pox and goat pox are dangerous diseases of domestic ruminants. Representatives of the genus of capripoxviruses are antigenically similar and can be used as a vaccine for three infections, as in the case of representatives of the genus of orthopoxviruses, which includes viruses of smallpox, monkeypox, and cowpox, that all belong to a single family Poxviridae.
In this study, the vaccine strain G20-LKV of the goat pox virus and the virulent strain RIBSP2019/K of the LSD virus were used. The experiments were carried out on clinically healthy cattle of the Kazakh White-headed breed, aged six to eight months. Virological and serological research methods were used in the work.
All immunized animals that received different doses of the vaccine showed resistance to the infection challenge, without showing any clinical signs of the disease. In animals that received the lowest doses of the vaccine 15,000, 30,000 and 40,000 TCID50, no adverse events, skin and temperature reactions were observed at the injection site. Those vaccinated with high doses of the vaccine had a local reaction in the form of swelling at the site of vaccine administration. Control animals infected with a virulent virus showed clinical signs of the cattle lumpy skin disease .
The vaccine, prepared based on the \"G20-LKV\" strain of the goat virus, is protective for cattle against infection with a virulent LSD virus at immunizing doses from 15,000 to 80,000 TCID50, which are dependent on the LSD epizootic situation in particular region.