■本研究旨在确定使用可育鸡蛋的异丙酚对小脑和脊髓的可能的胚胎毒性作用。
■将430个可育卵分为5组:对照组,盐水,2.5mg。kg-1,12.5mg。kg-1和37.5mg。kg-1异丙酚。在培育之前立即通过空气室进行注射。15日,18日,孵化的第21天,评价每组6个胚胎。取小脑和脊髓的连续石蜡切片用苏木精-伊红染色,克鲁弗-巴雷拉,甲苯胺蓝,和高碘酸-希夫反应。测量了外颗粒层和总皮层厚度,并测定了浦肯野细胞的线性密度。计算了灰质表面积与脊髓总表面积的比率。还评估了中管的横向和纵向直径。
■在宏观检查的任何胚胎中均未观察到结构畸形。在小脑和脊髓的发育和组织学组织方面,两组之间没有观察到显着差异。然而,在15号,18日,第21天,在不同剂量的丙泊酚组中,小脑的外颗粒层(所有天数p<0.001)和总皮质厚度(分别为p<0.01,p<0.001和p<0.001)显着不同程度地降低。同样,在脊髓中,灰质表面积与总表面积之比发生了显着变化(分别为p<0.01和p<0.001)。
■得出的结论是,在孵育前立即给予丙泊酚对发育中的小脑和脊髓有不利影响。因此,对于麻醉医生来说,在治疗育龄女性患者时始终保持警惕是很重要的。
UNASSIGNED: This study aims to determine the possible embryotoxic effects of propofol on the cerebellum and spinal cord using fertile chicken eggs.
UNASSIGNED: A total of 430 fertile eggs were divided into 5 groups: control, saline, 2.5 mg.kg-1, 12.5 mg.kg-1, and 37.5 mg.kg-1 propofol. Injections were made immediately before incubation via the air chamber. On the 15th, 18th, and 21st day of incubation, 6 embryos from each group were evaluated. Serial paraffin sections taken from the cerebellum and spinal cord were stained with hematoxylin-eosin, Kluver-Barrera, toluidine blue, and periodic acid-Schiff\'s reaction. The outer granular layer and total cortex thickness were measured, and the linear density of the Purkinje cells was determined. The ratios of the substantia grisea surface area to the total surface area of the spinal cord were calculated. The transverse and longitudinal diameters of the canalis centralis were also assessed.
UNASSIGNED: No structural malformation was observed in any embryos examined macroscopically. No significant difference was observed between the groups in terms of development and histologic organization of the cerebellum and spinal cord. However, on the 15th, 18th, and 21st day, the outer granular layer (p < 0.001 for all days) and the total cortex thickness (p < 0.01, p < 0.001, and p < 0.001, respectively) decreased significantly in different propofol dose groups in varying degrees in the cerebellum. Similarly, in the spinal cord, there were significant changes in the ratios of the substantia grisea surface area to the total surface area (p < 0.01 and p < 0.001, respectively).
UNASSIGNED: It was concluded that the in-ovo-administered propofol given immediately before incubation has adverse effects on the developing cerebellum and spinal cord. Therefore, it is important for anesthesiologists always to remain vigilant when treating female patients of childbearing age.