Abstract：
Drug use during pregnancy is an important issue that must be investigated due to its adverse effects on maternal and foetal health. This study aimed to determine the embryotoxic and teratogenic effects of in-ovo administered metamizole (dipyrone), which can be used when needed during pregnancy and has potent analgesic, antipyretic, anti-inflammatory, and long bone (tibia and femur) effects. This study used 240 fertile eggs from Atak S breed chickens, divided into eight equal groups: control, vehicle control, and 15.62, 31.25, 62.5, 125, 250 and 500 mg/kg metamizole. The eggs were hatched on the 21st day of incubation, and the chicks\' body weights and mortality rates were determined. The right and left femur and tibia bones were resected from the chicks. Anatomical reference points were determined after removing the soft tissues of the bones, and necessary morphometric measures were taken from these points with a 0.01 mm precision using digital callipers. The 100% lethal dose (LD100) was identified in the highest examined dose (500 mg/kg) in the Chicken Embryotoxicity Screening Test (CHEST)-I stage. The CHEST-II stage determined the 50% lethal dose (LD50). High-dose metamizole affected skeletal development, significantly decreasing tibia and femur lengths and corpus thicknesses and increasing mortality.
摘要：
怀孕期间的药物使用是一个重要问题，由于其对母婴健康的不利影响，必须进行调查。本研究旨在确定卵内给药美咪唑（dipyrone）的胚胎毒性和致畸作用，它可以在怀孕期间需要时使用，并具有有效的镇痛作用，退烧药,抗炎,和长骨（胫骨和股骨）的影响。这项研究使用了240个来自AtakS品种鸡的可育卵，分为八个相等的组：对照，车辆控制,和15.62、31.25、62.5、125、250和500mg/kg安乃近。卵在孵化的第21天孵化，并确定小鸡的体重和死亡率。从小鸡中切除左右股骨和胫骨。解剖参考点是在去除骨骼的软组织后确定的，并且使用数字卡尺以0.01mm的精度从这些点进行必要的形态测量。在鸡胚毒性筛选试验(CHEST)-I阶段的最高检查剂量(500mg/kg)中鉴定了100%致死剂量(LD100)。CHEST-II阶段确定了50％致死剂量（LD50）。高剂量安乃近影响骨骼发育，显著减少胫骨和股骨长度和体部厚度，增加死亡率。
