Abstract:
Pressure exerted by fluid contained within a lumen plays a crucial role in the growth, morphogenesis, and patterning of epithelial organs. Accurate modulation of lumen pressure in the developing embryo requires sensitive and robust methods that can detect and vary pressure in the range of tens to hundreds of Pascals (Pa). Here we describe a simple, cost-effective protocol for setting up a pressure modulation apparatus combining a high-sensitivity pressure sensor and a water column whose height can be finely tuned. We demonstrate lumen pressure control using the developing brain of early chicken embryos.
摘要:
管腔内流体施加的压力在生长中起着至关重要的作用,形态发生,和上皮器官的图案。发育中的胚胎中的管腔压力的精确调节需要灵敏且稳健的方法,该方法可以检测和改变数十到数百帕斯卡(Pa)范围内的压力。这里我们描述一个简单的,成本有效的协议,用于设置结合高灵敏度压力传感器和高度可以微调的水柱的压力调制装置。我们证明了使用早期鸡胚发育中的大脑来控制管腔压力。