PDF(Pubmed)
Abstract：
The late stage of embryo development is a crucial period of metabolic changes, with rapid organ development requiring a substantial supply of nutrients. During this phase, maternal nutritional levels play a vital role in the growth, development, and metabolism of the offspring. In this study, we added 2 doses of β-carotene (βc) (120 mg/kg and 240 mg/kg) to the daily diet of Hailan Brown laying hens to investigate the impact of maternal nutritional enrichment on embryo development. Maternal nutrition supplementation significantly increased the expression of chicken embryo liver index, growth hormone (GH), insulin-like growth factor-1 (IGF-1), and hepatocyte growth factor (HGF) in serum. At the same time, the expression of GH/growth hormone receptor (GHR), IGF-1 mRNA, and Proliferating Cell Nuclear Antigen (PCNA) protein in the liver was upregulated, indicating that maternal nutrition intervention may promote chicken embryo liver development through the GH-IGF-1 axis. Transcriptome sequencing results showed that differential genes in liver after maternal nutritional supplementation with β-carotene were enriched in pathways related to cell proliferation and metabolism. Consequently, we postulated that maternal β-carotene supplementation might operate via the GH-IGF-1 axis to regulate the expression of genes involved in growth and development, thereby promoting liver development. These results contribute to formulating more effective poultry feeding strategies to promote offspring growth and development.
摘要：
胚胎发育的后期是代谢改变的关键时期,快速的器官发育需要大量的营养供应。在这个阶段,母亲的营养水平在生长中起着至关重要的作用，发展,和后代的新陈代谢。在这项研究中,我们在海兰布朗蛋鸡的日常日粮中添加了2个剂量的β-胡萝卜素（βc）（120mg/kg和240mg/kg），以研究母体营养丰富对胚胎发育的影响。母亲补充营养显著提高了鸡胚肝脏指数的表达，生长激素（GH），胰岛素样生长因子-1（IGF-1），和血清中的肝细胞生长因子（HGF）。同时,GH/生长激素受体（GHR）的表达，IGF-1mRNA,肝脏中的增殖细胞核抗原（PCNA）蛋白上调，提示母体营养干预可能通过GH-IGF-1轴促进鸡胚肝脏发育。转录组测序结果表明，补充β-胡萝卜素后，母体肝脏中的差异基因富集在与细胞增殖和代谢相关的通路中。因此,我们推测母体补充β-胡萝卜素可能通过GH-IGF-1轴来调节生长和发育相关基因的表达，从而促进肝脏发育。这些结果有助于制定更有效的家禽饲养策略以促进后代的生长和发育。
