进行这项研究是为了分析使用微毛细管采血管和微血细胞比容管的儿科患者的指尖毛细血管采血,并比较通过这两种采血方法获得的血细胞分析结果。使用微毛细管采血管和微血细胞比容管从110名门诊患者收集手指毛细血管血,并使用SysmexXS-900i血液学分析仪和手动显微镜检查血细胞形态进行全血细胞计数分析。使用微血细胞比容样品作为参考组和来自微毛细管采血管的样品作为观察组,评估配对数据的一致性和偏倚性。两种血液收集方法在测量红细胞(RBC)参数方面表现出良好的一致性(即,红细胞,Hb,HCT,MCV,MCH和MCHC),其中相对偏差>0.91%的允许总误差(TEa),1.82%,11.82%,1.82%,0.91%和8.18%的参数措施,分别。根据行业要求,满足可接受偏倚水平的样本比例应>80%。此外,每个医学决策水平的估计偏差在RBC的临床可接受水平内,Hb,HCT,和MCV。然而,相对偏倚>TEa的WBC和PLT计数比例分别为25.45%和35.45%,分别。此外,医学决策水平为0.5×109/L时的白细胞计数和医学决策水平为10×109/L和50×109/L时的血小板计数的相对偏倚具有临床意义。Bland-Altman分析进一步显示,平均偏差为0.66×109/L(95%LoA,-0.79至2.11)的白细胞计数和39×109/L(95%LoA,-46至124),用于来自微毛细管采血管中收集的血液样品的PLT计数,与微血细胞比容管中收集的那些计数进行比较。中性粒细胞,单核细胞,淋巴细胞,嗜酸性粒细胞,与微血细胞比容管相比,微毛细管采血管中的PLT计数显着增加,仪器误报数量增加(P<0.05)。两种毛细管血液收集装置表现出性能差异。因此,临床医生应注意不同采血方法引起的结果差异。
This study was performed to analyze fingertip capillary blood sampling in pediatric patients using microcapillary blood collection tubes and microhematocrit tubes and to compare the blood cell analysis results obtained via these two blood collection methods. Finger capillary blood was collected from 110 outpatients using microcapillary blood collection tubes and microhematocrit tubes and complete blood count analysis was performed with a Sysmex XS-900i hematology analyzer and manual microscopy for blood cell morphology. Paired data was evaluated for agreement and bias using the microhematocrit samples as the reference group and the samples from the microcapillary blood collection tubes as the observation group. The two blood collection methods demonstrated good agreement for measuring red blood cell (RBC) parameters (i.e., RBC, Hb, Hct, MCV, MCH and MCHC), wherein the relative bias was > allowable total error (TEa) in 0.91%, 1.82%, 11.82%, 1.82%, 0.91% and 8.18% of the parameter measures, respectively. According to industry requirements, the proportion of samples meeting the acceptable bias level should be > 80%. Additionally, the estimated biases at each medical decision level were within clinically acceptable levels for RBC, Hb, Hct, and MCV. However, the proportion of WBC and PLT counts with relative bias > TEa was 25.45% and 35.45%, respectively. Furthermore, the relative bias of the WBC count at the medical decision level of 0.5 × 109/L and that of the PLT counts at the medical decision levels of 10 × 109/L and 50 × 109/L were clinically significant. Bland-Altman analysis further showed a mean bias of 0.66 × 109/L (95% LoA, - 0.79 to 2.11) for the WBC count and 39 × 109/L (95% LoA, - 46 to 124) for the PLT count from the blood samples collected in the microcapillary blood collection tubes compared with the counts of those collected in the microhematocrit tubes. Neutrophil, monocyte, lymphocyte, eosinophil, and PLT counts increased significantly in the microcapillary blood collection tubes compared with those in the microhematocrit tubes, along with an elevated number of instrument false alarms (P < 0.05). The two capillary blood collection devices exhibit performance differences. Therefore, clinicians should pay attention to the variation in results caused by different blood collection methods.