Abstract：
A comparative study of the morphological and functional state of the microvasculature of the substantia nigra pars compacta of the brain (SNc) and bone marrow of rats was carried out using the rotenone model of Parkinson\'s disease (PD) and with subsequent administration of bacterial melanin (BM). The detection of microvasculature was carried out according to the histoangiological method of Chilingaryan. Animal behavior was studied using a cylinder test. An analysis of morphometric data showed that, in comparison with control animals, experimental animals with rotenone dysfunction showed an increase in capillary diameters and a general reduction in the capillary link in SNc. Behavioral tests have shown that the animals with rotenone intoxication exhibit a form of behavior inherent in PD (freezing, immobility, apathy). Under the influence of BM, the diameter of the capillaries in the SNc approaches the norm, and the capillary link is restored. Due to the protective effect of BM in rats with rotenone intoxication, the trophism of the brain tissue increases as a result of the approach of the lumen of the vessels to the norm and the opening of new branches in the capillary network, an increase in the density of capillaries, which ensures the safety of nerve cells. Animal behavior indicators are close to normal. A comprehensive analysis of cytogenetic data of rat bone marrow was also carried out. In animals with PD, compared to controls, there is a significant increase in the amount of polyploid cells (PC) and a decrease in the level of mitotic index (MI), which usually manifests itself in inflammatory processes and is accompanied by inhibition of bone marrow hematopoiesis. Under the influence of BM, a tendency towards normalization of MI was noted and a significant decrease in the percentage of PC was obtained, which possibly indicates its beneficial effect. The data obtained suggest that BM can be used as a therapeutic agent in the treatment of PD.
摘要：
使用帕金森氏病（PD）的鱼藤酮模型并随后施用细菌黑色素（BM），对大鼠的脑黑质（SNc）和骨髓的微血管的形态和功能状态进行了比较研究。根据Chilingaryan的组织血管学方法进行微血管系统的检测。使用圆筒试验研究动物行为。对形态测量数据的分析表明,与对照动物相比，鱼藤酮功能障碍的实验动物显示出毛细血管直径的增加和SNc中毛细血管连接的普遍减少。行为测试表明，鱼藤酮中毒的动物表现出PD固有的行为形式（冷冻，不动，冷漠）。在BM的影响下,SNc中毛细血管的直径接近标准，毛细管连接恢复。由于BM对鱼藤酮中毒大鼠的保护作用，由于血管腔接近常态和毛细血管网络中新分支的开放，脑组织的营养增加，毛细血管密度的增加，确保神经细胞的安全。动物行为指标接近正常。还对大鼠骨髓的细胞遗传学数据进行了综合分析。在有PD的动物中，与对照组相比，多倍体细胞（PC）的数量显着增加，有丝分裂指数（MI）的水平降低，通常表现在炎症过程中，并伴有骨髓造血的抑制。在BM的影响下,注意到MI正常化的趋势，并获得PC百分比的显着降低，这可能表明它的有益效果。获得的数据表明BM可以用作治疗PD的治疗剂。
