单层离心(SLC)通过低密度胶体为抗生素在公猪精液补充剂中的使用提供了替代解决方案,与高密度胶体相比,成本较低。本研究的目的是探讨使用SLC制备的精液剂量而不使用抗生素时母猪的繁殖性能。在泰国的一家商业猪群中,采用低密度Porcicoll为人工授精准备精液剂量。射精被分成两个相等的部分来产生授精剂量,每剂含3000×106精子/80毫升,用于个别母猪的子宫内授精。母猪被授精两次,两次授精之间的间隔为8至16小时。对照组包括206剂接受抗生素治疗的精液,为103头母猪的授精做好准备,而SLC组包括194个SLC制备的精液剂量,不含抗生素,用于给97头母猪授精。生育力和繁殖力特征,包括无回报率,受孕率,分娩率,和凋落物性状(即,每窝出生的仔猪总数,每窝活着出生的仔猪数量,死产仔猪的数量,和木乃伊胎儿的数量),进行组间比较。此外,仔猪特性数据,包括活产和死产仔猪(即,死产的患病率(是的,no),出生体重,皇冠臀部长度,体重指数(BMI),和黄体指数(PI)),决心。无回报率无显著差异(75.7%与77.3%),受胎率(73.8%vs.73.2%),和分娩率(71.8%与73.2%)在对照组和SLC组之间观察到,分别为(P>0.05)。然而,SLC组每窝出生的仔猪总数高于对照组(14.6±0.9vs.分别为12.3±0.6,P=0.049)。有趣的是,SLC组的死产患病率低于对照组(6.2%vs.11.6%,分别,P<0.001)。此外,与对照组相比,SLC组的新生仔猪表现出更高的出生体重和BMI(1.36±0.03vs.1.26±0.02kg,P=0.005,18.3±0.3vs.17.3±0.2kg/m2,P=0.003)。总之,在商业育种操作中的人工授精中,通过低密度胶体在SLC后使用精子剂量不会对生育力或繁殖力特征产生不利影响,但在增加每窝出生的仔猪总数方面显示出潜在的益处。此外,观察到仔猪出生体重和身体指数的改善,死产的比例降低了。我们的发现为精液补充剂中的抗生素替代品提供了新的可能性,以降低养猪业中抗生素耐药性的风险。此外,它们提供了令人信服的生殖结局,支持将SLC准备的精液剂量整合到人工授精实践中.
Single Layer Centrifugation (SLC) through a low density colloid offers an alternative solution to antibiotic use in
boar semen extenders, with lower costs compared to high density colloids. The aim of this study was to explore the reproductive performance of sows when using SLC-prepared semen doses without antibiotics, employing low density Porcicoll to prepare semen doses for artificial insemination in a commercial swine herd in Thailand. Ejaculates were divided into two equal parts to create insemination doses, with each dose containing 3000 × 106 sperm/80 ml for intra-uterine insemination in individual sows. The sows were inseminated twice, with the interval between the two inseminations ranging from 8 to 16 h. The CONTROL group consisted of 206 semen doses treated with antibiotics, prepared for insemination in 103 sows, while the SLC group comprised 194 SLC-prepared semen doses without antibiotics for inseminating 97 sows. Fertility and fecundity traits, including non-return rate, conception rate, farrowing rate, and litter traits (i.e., the total number of piglets born per litter, number of piglets born alive per litter, number of stillborn piglets, and number of mummified fetuses), were compared between groups. Furthermore, data on piglet characteristics, including live-born and stillborn piglets (i.e., the prevalence of stillbirth (yes, no), birth weight, crown-rump length, body mass index (BMI), and ponderal index (PI)), were determined. No significant differences in non-return rate (75.7 % vs. 77.3 %), conception rate (73.8 % vs. 73.2 %), and farrowing rate (71.8 % vs. 73.2 %) were observed between the CONTROL and SLC groups, respectively (P > 0.05). Nevertheless, the total number of piglets born per litter in the SLC group was higher than in the CONTROL group (14.6 ± 0.9 vs. 12.3 ± 0.6, respectively, P = 0.049). Interestingly, the prevalence of stillbirth in the SLC group was lower than in the CONTROL group (6.2 % vs. 11.6 %, respectively, P < 0.001). Moreover, the newborn piglets in the SLC group exhibited higher birth weight and BMI compared to those in the CONTROL group (1.36 ± 0.03 vs. 1.26 ± 0.02 kg, P = 0.005, and 18.3 ± 0.3 vs. 17.3 ± 0.2 kg/m2, P = 0.003). In conclusion, employing sperm doses after SLC through a low density colloid in artificial insemination within a commercial breeding operation did not have a detrimental impact on either fertility or fecundity traits but showed potential benefits in increasing the total number of piglets born per litter. Moreover, improvements were observed in the birth weight and body indexes of piglets, and the percentage of stillbirths was reduced. Our findings introduce new possibilities for antibiotic alternatives in semen extenders to reduce the risk of antimicrobial resistance in the swine industry. Additionally, they provide compelling reproductive outcomes supporting the integration of SLC-prepared semen doses into artificial insemination practices.