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Abstract：
In this study, we investigated the correlation between the composition and function of the gut microbiota and the semen quality of Rongchang boars. Significant differences in gut microbial composition between boars with high (group H) and low (group L) semen utilization rates were identified through 16S rRNA gene sequencing, with 18 differential microbes observed at the genus level. Boars with lower semen utilization rates exhibited a higher relative abundance of Treponema, suggesting its potential role in reducing semen quality. Conversely, boars with higher semen utilization rates showed increased relative abundances of Terrisporobacter, Turicibacter, Stenotrophomonas, Clostridium sensu stricto 3, and Bifidobacterium, with Stenotrophomonas and Clostridium sensu stricto 3 showing a significant positive correlation with semen utilization rates. The metabolomic analyses revealed higher levels of gluconolactone, D-ribose, and 4-pyridoxic acid in the H group, with 4 pyridoxic acid and D-ribose showing a significant positive correlation with Terrisporobacter and Clostridium sensu stricto 3, respectively. In contrast, the L group showed elevated levels of D-erythrose-4-phosphate, which correlated negatively with Bifidobacterium and Clostridium sensu stricto 3. These differential metabolites were enriched in the pentose phosphate pathway, vitamin B6 metabolism, and antifolate resistance, potentially influencing semen quality. These findings provide new insights into the complex interplay between the gut microbiota and boar reproductive health and may offer important information for the discovery of disease biomarkers and reproductive health management.
摘要：
在这项研究中,研究了荣昌公猪肠道菌群组成和功能与精液质量的相关性。通过16SrRNA基因测序鉴定精液利用率高(H组)和低(L组)公猪之间肠道微生物组成的显著差异,在属水平上观察到18种差异微生物。精液利用率较低的公猪表现出较高的密螺旋体相对丰度，提示其在降低精液质量方面的潜在作用。相反，精液利用率较高的公猪显示出三孢菌的相对丰度增加，Turicibacter,窄食单胞菌,严格的感觉梭菌3和双歧杆菌，狭窄单胞菌和严格梭菌3与精液利用率呈显着正相关。代谢组学分析显示葡萄糖酸内酯水平较高，D-核糖,H组的4-吡啶酮酸，其中4个吡啶酮酸和D-核糖分别与三叶草杆菌和严格梭菌3呈显著正相关。相比之下,L组显示D-赤藓-4-磷酸水平升高，与双歧杆菌和严格的梭菌3呈负相关。这些差异代谢物富集在磷酸戊糖途径中，维生素B6代谢，和抗叶酸药物抗性，可能影响精液质量。这些发现为肠道微生物群与公猪生殖健康之间复杂的相互作用提供了新的见解，并可能为发现疾病生物标志物和生殖健康管理提供重要信息。
