{Reference Type}: Journal Article
{Title}: Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen.
{Author}: He M;Sun L;Xu J;Wu C;Zhang S;Gao J;Zhang D;Gan Y;Bian Y;Wei J;Zhang W;Zhang W;Han X;Dai J;
{Journal}: Animals (Basel)
{Volume}: 14
{Issue}: 10
{Year}: 2024 May 10
{Factor}: 3.231
{DOI}: 10.3390/ani14101422
{Abstract}: To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.