PDF(Pubmed)
Abstract：
To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
摘要：
为了解决使用LN运输公猪精液带来的安全问题，这项研究是对冷冻公猪精液在干冰（-79°C）中的短期储存进行的。将在LN中冷冻的公猪精液转移到干冰中，保存1天,3天,5天,7天,或8天，然后搬回LN.确定在LN或干冰中储存的冷冻精液的质量，以评估用干冰进行短距离运输的可行性。结果表明，60℃8s是冻精在干冰中解冻的最佳条件。精子活力无显著差异，质膜完整性，与LN相比，在干冰中短期储存后的精液中观察到顶体完整性（p>0.05）。储存组之间的抗氧化性能也没有显着变化（p>0.05）。总之,干冰可以作为冷冻猪精液短期运输至少7天的冷源，在不影响精子活力的情况下，形态完整性,或抗氧化剂指数。
