■方法异质性阻碍了肌腱和韧带特性的孤立研究中的数据比较,限制临床理解并影响替代材料的开发和评估。
■要在形态学上创建开放访问数据集,生物力学,和临床上重要的下肢肌腱和韧带的生化特性,使用一致的方法,能够直接比较肌腱/韧带。
■描述性实验室研究。
■从8只新鲜冷冻的人类尸体中检索到19种不同的下肢肌腱和韧带(5只雄性,3名女性;49-65岁)包括跟腱,胫骨后肌,胫骨前肌,腓骨(腓骨)长,腓骨(腓骨)短,幻觉长屈肌,幻长伸肌,plantaris,趾长屈肌,股四头肌,髌骨,半腱肌,和股筋;前十字交叉,后交叉,内侧侧支,和外侧副韧带;和10毫米宽的移植物来自对侧股四头肌和髌骨肌腱。结果包括形态学(组织长度,超声量化横截面积[CSAUS],以及主轴和副轴),生物力学(失效载荷,极限抗拉强度[UTS],失效应变,和弹性模量),和生物化学(硫酸化糖胺聚糖[sGAG]和羟脯氨酸含量)。使用混合模型回归分析组织差异。
■在不同的结果中,肌腱和韧带之间存在一系列的异同。与潜在移植组织适用性相关的一个关键发现是可比的失效载荷,UTS,CSAUS,sGAG,和羟脯氨酸存在于腿筋肌腱(标准移植源)和5条通常不用于移植的肌腱之间:腓骨(腓骨)长和短,长屈屈肌和长伸肌,和屈指长肌腱。
■这项对下肢肌腱和韧带的研究能够直接比较形态学,生物力学,和生化人体组织特性-选择合适的移植组织的关键因素。该分析确定了6种潜在的新供体组织,其特性与当前使用的移植物相当。
■这个广泛的数据集减少了利用来自不兼容来源的数据的需要,这可能有助于手术决定(例如,扩大被认为适合用作移植物的肌腱范围的证据),并可能为新的生物材料和计算模型提供一致的设计输入。已提供完整的数据集以方便进一步调查,具有扩展资源以包括其他结果和组织的能力。
UNASSIGNED: Methodological heterogeneity hinders data comparisons across isolated studies of tendon and ligament properties, limiting clinical understanding and affecting the development and evaluation of replacement materials.
UNASSIGNED: To create an open-access data set on the morphological, biomechanical, and biochemical properties of clinically important tendons and ligaments of the lower limb, using consistent methodologies, to enable direct tendon/ligament comparisons.
UNASSIGNED: Descriptive laboratory study.
UNASSIGNED: Nineteen distinct lower limb tendons and ligaments were retrieved from 8 fresh-frozen human cadavers (5 male, 3 female; aged 49-65 years) including Achilles, tibialis posterior, tibialis anterior, fibularis (peroneus) longus, fibularis (peroneus) brevis, flexor hallucis longus, extensor hallucis longus, plantaris, flexor digitorum longus, quadriceps, patellar, semitendinosus, and gracilis tendons; anterior cruciate, posterior cruciate, medial collateral, and lateral collateral ligaments; and 10 mm-wide grafts from the contralateral quadriceps and patellar tendons. Outcomes included morphology (tissue length, ultrasound-quantified cross-sectional area [CSAUS], and major and minor axes), biomechanics (failure load, ultimate tensile strength [UTS], failure strain, and elastic modulus), and biochemistry (sulfated glycosaminoglycan [sGAG] and hydroxyproline contents). Tissue differences were analyzed using mixed-model regression.
UNASSIGNED: There was a range of similarities and differences between tendons and ligaments across outcomes. A key finding relating to potential graft tissue suitability was the comparable failure loads, UTS, CSAUS, sGAG, and hydroxyproline present between hamstring tendons (a standard graft source) and 5 tendons not typically used for grafting: fibularis (peroneus) longus and brevis, flexor and extensor hallucis longus, and flexor digitorum longus tendons.
UNASSIGNED: This study of lower limb tendons and ligaments has enabled direct comparison of morphological, biomechanical, and biochemical human tissue properties-key factors in the selection of suitable graft tissues. This analysis has identified 6 potential new donor tissues with properties comparable to currently used grafts.
UNASSIGNED: This extensive data set reduces the need to utilize data from incompatible sources, which may aid surgical decisions (eg, evidence to expand the range of tendons considered suitable for use as grafts) and may provide congruent design inputs for new biomaterials and computational models. The complete data set has been provided to facilitate further investigations, with the capacity to expand the resource to include additional outcomes and tissues.