农业废物是生物资源利用不足以及缺乏以适当方式或循环经济方法重新利用这种废物的知识的结果。在印度的医疗系统中,Cyperusscariosus(CS)的根由于其重要的药用特性而被大规模使用。不幸的是,CS的空中部分被视为农业废物,是未充分利用的生物资源。由于缺乏知识,CS被视为杂草。本研究是首次尝试探索CS叶作为药用和营养丰富的来源。确定被忽略部分的莎草R.Br.的食物和营养价值。(CS),即CS叶,通过新开发的HPLC和基于ICPOES的方法对CS的植物化学物质和金属离子进行定量。在咖啡酸的HPLC分析中观察到的植物化学物质的含量,儿茶素,表儿茶素,反式-对-香豆酸,反式阿魏酸为10.51、276.15、279.09、70.53和36.83µg/g,分别。在GC-MS/MS分析中,脂肪酸包括亚麻酸,植物醇,棕榈酸,等。已确定。在ICPOES分析中,Na的显著含量,K,Ca,Cu,Fe,Mg,Mn,并观察到Zn。CS叶的TPC和TFC为17.933mgGAEq。/g和130.767mgQCEeq。与标准品(CaCO3)相比,在DPPH测定中以及2.78mg/mL的IC50值和更好的抗酸活性。CS叶的甲醇提取物对金黄色葡萄球菌(15±2mm)具有抗微生物活性,铜绿假单胞菌(12±2mm)和大肠杆菌(10±2mm)。计算机模拟研究证实了从抗氧化剂获得的体外结果,抗酸剂,和抗微生物研究。此外,计算机模拟研究揭示了CS叶的抗癌和抗炎潜力。这项研究,因此,证明了CS未充分利用部分的药用意义,以及将农业废物转化为人类活性的药物有效材料。因此,本研究强调,CS叶具有良好的营养效用,具有重要的药用价值,在制药行业具有巨大的潜力,以及改善生物价值和环境。
Agro-waste is the outcome of the under-utilization of bioresources and a lack of knowledge to re-use this waste in proper ways or a circular economy approach. In the Indian medicinal system, the root of Cyperus scariosus (CS) is used at a large scale due to their vital medicinal properties. Unfortunately, the aerial part of CS is treated as agro-waste and is an under-utilized bioresource. Due to a lack of knowledge, CS is treated as a weed. This present study is the first ever attempt to explore CS leaves as medicinally and a nutrient rich source. To determine the food and nutritional values of the neglected part of Cyperus scariosus R.Br. (CS), i.e. CS leaves, phytochemicals and metal ions of CS were quantified by newly developed HPLC and ICPOES-based methods. The content of the phytochemicals observed in HPLC analysis for caffeic acid, catechin, epicatechin, trans-p-coumaric acid, and trans-ferulic acid was 10.51, 276.15, 279.09, 70.53, and 36.83 µg/g, respectively. In GC-MS/MS analysis, fatty acids including linolenic acid, phytol, palmitic acid, etc. were identified. In ICPOES analysis, the significant content of Na, K, Ca, Cu, Fe, Mg, Mn, and Zn was observed. The TPC and TFC of the CS leaves was 17.933 mg GAE eq./g and 130.767 mg QCE eq./g along with an IC50 value of 2.78 mg/mL in the DPPH assay and better
antacid activity was measured than the standard (CaCO3). The methanolic extract of CS leaves showed anti-microbial activity against Staphylococcus aureus (15 ± 2 mm), Pseudomonas aeruginosa (12 ± 2 mm) and Escherichia coli (10 ± 2 mm). In silico studies confirmed the in vitro results obtained from the antioxidant, antiacid, and anti-microbial studies. In addition, in silico studies revealed the anti-cancerous and anti-inflammatory potential of the CS leaves. This study, thus, demonstrated the medicinal significance of the under-utilized part of CS and the conversion of agro-waste into mankind activity as a pharmaceutical potent material. Consequently, the present study highlighted that CS leaves have medicinal importance with good nutritional utility and have a large potential in the pharmaceutical industry along with improving bio-valorization and the environment.