PDF(Pubmed)
Abstract：
In the current study, Lactobacillus acidophilus was encapsulated in sodium alginate and whey protein isolate, with the addition of antacids CaCO3 or Mg(OH)2. The obtained microgels were observed by scanning electron microscopy. Encapsulated and free probiotics were subjected to vitality assay under stressed conditions. Furthermore, dried apple snack was evaluated as a carrier for probiotics for 28 days. A significant (p ≤ .05) effect of antacid with an encapsulating agent was observed under different stressed conditions. During exposure to simulated gastrointestinal conditions, there were observations of 1.24 log CFU and 2.17 log CFU, with corresponding 0.93 log CFU and 2.63 log CFU decrease in the case of SA + CaCO3 and WPI + CaCO3 respectively. Likewise, high viability was observed under thermal and refrigerated conditions for probiotics encapsulated with SA + CaCO3. In conclusion, the results indicated that alginate microgels with CaCO3 are effective in prolonging the viability of probiotics under stressed conditions.
摘要：
在目前的研究中,嗜酸乳杆菌被包裹在海藻酸钠和乳清分离蛋白中，添加抗酸剂CaCO3或Mg(OH)2。通过扫描电子显微镜观察获得的微凝胶。在应激条件下对封装的和游离的益生菌进行活力测定。此外，将干苹果零食作为益生菌的载体评价28天。在不同的应力条件下观察到抗酸剂与包封剂的显着（p≤0.05）作用。在暴露于模拟胃肠道条件期间，有1.24对数CFU和2.17对数CFU的观测值，在SA+CaCO3和WPI+CaCO3的情况下，相应的0.93logCFU和2.63logCFU分别降低。同样，在热和冷藏条件下观察到用SA+CaCO3包封的益生菌的高活力。总之,结果表明，含CaCO3的藻酸盐微凝胶可有效延长应激条件下益生菌的活力。
